Multi-Walled Carbon Nanotubes Improved Development during In Vitro Multiplication of Sugarcane (Saccharum spp.) in a Semi-Automated Bioreactor
| Autor: | José Luis Spinoso-Castillo, Fernando Carlos Gómez-Merino, Monserrat Sorcia-Morales, Jericó J. Bello-Bello, Lino Sánchez-Segura |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_element
Plant Science Carbon nanotube carbon content law.invention Saccharum chemistry.chemical_compound macro- and micronutrients law hormesis in vitro culture Bioreactor Dry matter chlorophyll Ecology Evolution Behavior and Systematics Ecology biology Chemistry Botany food and beverages biology.organism_classification Horticulture Chlorophyll QK1-989 Shoot Carbon Explant culture |
| Zdroj: | Plants, Vol 10, Iss 2015, p 2015 (2021) Plants Volume 10 Issue 10 |
| ISSN: | 2223-7747 |
| Popis: | Carbon nanotubes play an important role in plant biotechnology due to their effects on the growth and differentiation of cells, tissues, organs, and whole plants. This study aimed to evaluate the effect of multi-walled carbon nanotubes (MWCNTs) during in vitro multiplication of sugarcane (Saccharum spp.) using a temporary immersion system. Morphological characterization of MWCNTs was carried out under a transmission electron microscope. Different concentrations (0, 50, 100, 200 mg L−1) of MWCNTs were added to Murashige and Skoog liquid culture medium in the multiplication stage. At 30 d of culture, number of shoots per explant, shoot length, number of leaves per shoot, total chlorophyll, dry matter percentage, carbon percentage, and macro- and micronutrient content were evaluated. Results showed an increase in the development of sugarcane shoots at concentrations of 100 and 200 mg L−1 MWCNT. Total chlorophyll content increased at concentrations of 50 and 100 mg L−1 MWCNT, whereas macro- and micronutrient content was variable at the different MWCNT concentrations. Results suggest a hormetic effect, characterized by stimulation at low concentrations. In conclusion, the use of low concentrations of MWCNTs had positive effects on development, total chlorophyll, carbon percentage, and macro- and micronutrient (N, Ca, S, Fe, Cu, Zn and Na) contents during in vitro multiplication of sugarcane and may have a potential use in other species of agricultural interest. |
| Databáze: | OpenAIRE |
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