The Plant Defense Elicitor Cryptogein Stimulates Clathrin-Mediated Endocytosis Correlated with Reactive Oxygen Species Production in Bright Yellow-2 Tobacco Cells
| Autor: | Nathalie Leborgne-Castel, Valérie Houot, Jeannine Lherminier, Jérôme Fromentin, Françoise Simon-Plas, Christophe Der |
|---|---|
| Přispěvatelé: | Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD) |
| Rok vydání: | 2008 |
| Předmět: |
0106 biological sciences
Physiology media_common.quotation_subject Plant Science Endocytosis 01 natural sciences Clathrin [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics Cell membrane 03 medical and health sciences Genetics medicine DEFENSE DES PLANTES Internalization 030304 developmental biology media_common 0303 health sciences NADPH oxidase biology Receptor-mediated endocytosis FORMES ACTIVES DE L'OXYGENE Cell biology Elicitor medicine.anatomical_structure REACTIONS DE DEFENSE biology.protein Signal transduction 010606 plant biology & botany |
| Zdroj: | Plant Physiology Plant Physiology, American Society of Plant Biologists, 2008, 146, pp.1255-1266. ⟨10.1104/pp.107.111716⟩ |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.107.111716 |
| Popis: | The plant defense elicitor cryptogein triggers well-known biochemical events of early signal transduction at the plasma membrane of tobacco (Nicotiana tabacum) cells, but microscopic observations of cell responses related to these early events were lacking. We determined that internalization of the lipophilic dye FM4-64, which is a marker of endocytosis, is stimulated a few minutes after addition of cryptogein to tobacco Bright Yellow-2 (BY-2) cells. This stimulation is specific to the signal transduction pathway elicited by cryptogein because a lipid transfer protein, which binds to the same receptor as cryptogein but without triggering signaling, does not increase endocytosis. To define the nature of the stimulated endocytosis, we quantified clathrin-coated pits (CCPs) forming on the plasma membrane of BY-2 cells. A transitory stimulation of this morphological event by cryptogein occurs within the first 15 min. In the presence of cryptogein, increases in both FM4-64 internalization and clathrin-mediated endocytosis are specifically blocked upon treatment with 5 μ m tyrphostin A23, a receptor-mediated endocytosis inhibitor. The kinetics of the transient increase in CCPs at the plasma membrane coincides with that of transitory reactive oxygen species (ROS) production occurring within the first 15 min after elicitation. Moreover, in BY-2 cells expressing NtrbohD antisense cDNA, which are unable to produce ROS when treated with cryptogein, the CCP stimulation is inhibited. These results indicate that the very early endocytic process induced by cryptogein in tobacco is due, at least partly, to clathrin-mediated endocytosis and is dependent on ROS production by the NADPH oxidase NtrbohD. |
| Databáze: | OpenAIRE |
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