Human platelet-rich plasma stimulates migration and chondrogenic differentiation of human subchondral progenitor cells
| Autor: | Jan Philipp Krüger, Axel Pruss, Christian Kaps, Alberto Siclari, Sylvia Hondke, Michaela Endres |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Osteocalcin Type II collagen Gene Expression Fatty Acid-Binding Proteins Chondrocyte Chondrocytes Cell Movement medicine Humans Orthopedics and Sports Medicine Progenitor cell Collagen Type II Cells Cultured Aged biology Platelet-Rich Plasma Chemotaxis Cell Differentiation Mesenchymal Stem Cells Middle Aged Chondrogenesis Culture Media Cell biology medicine.anatomical_structure Adipogenesis Platelet-rich plasma Immunology biology.protein Proteoglycans Stem cell Cell Migration Assays |
| Zdroj: | Journal of Orthopaedic Research. 30:845-852 |
| ISSN: | 0736-0266 |
| Popis: | In cartilage repair, platelet-rich plasma (PRP) is used in one-step approaches utilizing microfracture and matrix-induced chondrogenesis procedures, bone marrow-derived cell transplantation, or intra-articular injection. The aim of our study was to evaluate the effect of human PRP on the migration and chondrogenic differentiation of human subchondral progenitors. Human progenitors were derived from subchondral cortico-spongious bone (CSP), were analyzed for their migration capacity upon PRP treatment in 96-well chemotaxis assays and cultured in high-density pellet cultures under serum-free conditions in the presence of 5% PRP. Chemotaxis assays showed that 0.1–100% PRP significantly (p |
| Databáze: | OpenAIRE |
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