Characterization of four lipoprotein classes in human cerebrospinal fluid
| Autor: | Miriam Kreckel, Ulrike Beisiegel, Nicolette Donarski, Carsten Buhmann, Hans-Joerg Stuerenburg, Stefanie Koch, Kathrin Goetze |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Apolipoprotein E medicine.medical_specialty Apolipoprotein B Lipoproteins Blotting Western QD415-436 Biochemistry Chromatography Affinity affinity chromatography chemistry.chemical_compound Apolipoproteins E Endocrinology Cerebrospinal fluid Affinity chromatography Internal medicine medicine Humans Phospholipids apolipoprotein E Apolipoprotein A-I biology Cholesterol Fatty Acids Cell Biology Blot Microscopy Electron chemistry Chromatography Gel biology.protein Female lipids (amino acids peptides and proteins) Antibody Lipoprotein |
| Zdroj: | Journal of Lipid Research, Vol 42, Iss 7, Pp 1143-1151 (2001) |
| ISSN: | 0022-2275 |
| DOI: | 10.1016/s0022-2275(20)31605-9 |
| Popis: | Lipoprotein metabolism in brain has not yet been fully elucidated, although there are a few reports concerning lipids in the brain and lipoproteins and apolipoproteins in the cerebrospinal fluid (CSF). To establish normal levels of lipoproteins in human CSF, total cholesterol, phospholipids, and fatty acids as well as apolipoprotein E (apoE) and apoA-I levels were determined in CSF samples from 216 individuals. For particle characterization, lipoproteins from human CSF were isolated by affinity chromatography and analyzed for size, lipid and apolipoprotein composition. Two consecutive immunoaffinity columns with antibodies, first against apoE and subsequently against apoA-I, were used to define four distinct lipoprotein classes. The major lipoprotein fraction consisted of particles of 13–20 nm containing apoE and apoA-I as well as apoA-IV, apoD, apoH, and apoJ. In the second particle class (13–18 nm) mainly apoA-I and apoA-II but no apoE was detected. Third, there was a small number of large particles (18–22 nm) containing no apoA-I but apoE associated with apoA-IV, apoD, and apoJ. In the unbound fraction we detected small particles (10–12 nm) with low lipid content containing apoA-IV, apoD, apoH, and apoJ.In summary, we established lipid and apolipoprotein levels in CSF in a large group of individuals and described four distinct lipoprotein classes in human CSF, differing in their apolipoprotein pattern, lipid composition, and size. On the basis of our own data and previous findings from other groups, we propose a classification of CSF lipoproteins.—Koch, S., N. Donarski, K. Goetze, M. Kreckel, H-J. Stuerenburg, C. Buhmann, and U. Beisiegel. Characterization of four lipoprotein classes in human cerebrospinal fluid. J. Lipid Res. 2001. 42: 1143–1151. |
| Databáze: | OpenAIRE |
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