Improved synaptic and cognitive function in aged 3 × Tg-AD mice with reduced amyloid-β after immunotherapy with a novel recombinant 6Aβ15-TF chimeric vaccine
| Autor: | Yun-Zhou Yu, Xiaobin Pang, Pei-Tang Huang, Qing Xu, Si Liu, Qing-Li Li, Xiao-Wei Zhou, Hai-Chao Wang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Aging Alzheimer Vaccines medicine.medical_treatment Protein subunit Recombinant Fusion Proteins Immunology Mice Transgenic Neuroprotection Protein Aggregation Pathological law.invention 03 medical and health sciences Mice 0302 clinical medicine Immune system Cognition Electrical Synapses Antigen law Alzheimer Disease medicine Immunology and Allergy Animals Humans Amyloid beta-Peptides biology Behavior Animal business.industry Immunogenicity Immunotherapy Peptide Fragments Immunity Humoral Mice Inbred C57BL Disease Models Animal 030104 developmental biology biology.protein Recombinant DNA Female Immunization Antibody business 030217 neurology & neurosurgery |
| Zdroj: | Clinical immunology (Orlando, Fla.). 193 |
| ISSN: | 1521-7035 |
| Popis: | Alzheimer's disease (AD) is the most common progressive neurodegenerative disorder impairing memory and cognition. In this study, we describe the immunogenicity and protective efficacy of the novel recombinant 6Aβ15-TF chimeric antigen as a subunit protein vaccine for AD. Recombinant 6Aβ15-TF chimeric vaccine induced strong Aβ-specific humoral immune responses without Aβ-specific T cell immunity in C57/BL6 and 3 × Tg-AD mice at different ages. As an early immunotherapy model for AD, this vaccine induced high titers of long-lasting anti-Aβ42 antibodies in aged 3 × Tg-AD mice, which led to improve behavioral performance and markedly reduced the levels of insoluble and soluble Aβ and Aβ oligomers. In agreement with these findings, immunotherapy with 6Aβ15-TF prevented the Aβ-induced decrease of presynaptic and postsynaptic proteins in aged 3 × Tg-AD mice. Our results suggest that this novel and highly immunogenic recombinant 6Aβ15-TF chimeric vaccine provides neuroprotection in AD mice and can be considered an effective AD candidate vaccine. |
| Databáze: | OpenAIRE |
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