Cryo‐EM structure of the ribosome functional complex of the human pathogen Staphylococcus aureus at 3.2 Å resolution

Autor: Bulat Fatkhullin, Marat Yusupov, Shamil Validov, Azat Gabdulkhakov, Iskander Khusainov, Konstantin S. Usachev, Lasse Jenner, Alexander Golubev, Gulnara Yusupova
Přispěvatelé: Centre National de la Recherche Scientifique (CNRS), Kazan Federal University (KFU), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institute of Protein Research, Russian Academy of Sciences [Moscow] (RAS), Max-Planck-Institut für Biophysik - Max Planck Institute of Biophysics (MPIBP), Max-Planck-Gesellschaft, Yusupova, Gulnara
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Models
Molecular
Staphylococcus aureus
antibiotic resistance
[SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry
Molecular Biology/Structural Biology [q-bio.BM]
medicine.drug_class
methyltransferases
[SDV]Life Sciences [q-bio]
Antibiotics
Biophysics
Human pathogen
Biology
Ligands
medicine.disease_cause
Biochemistry
Ribosome
Microbiology
rRNA modifications
03 medical and health sciences
Antibiotic resistance
RNA
Transfer
Structural Biology
RNA
Ribosomal
16S
drug targets
Genetics
medicine
RNA
Messenger
Molecular Biology
Pathogen
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
0303 health sciences
Messenger RNA
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Structural Biology [q-bio.BM]
Cryoelectron Microscopy
030302 biochemistry & molecular biology
Reproducibility of Results
Cell Biology
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
3. Good health
RNA
Ribosomal
23S
Transfer RNA
[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Ribosomes
Zdroj: FEBS Letters
FEBS Letters, Wiley, 2020, ⟨10.1002/1873-3468.13915⟩
FEBS Letters, 2020, 594 (21), pp.3551-3567. ⟨10.1002/1873-3468.13915⟩
FEBS Letters, Wiley, 2020, 594 (21), pp.3551-3567. ⟨10.1002/1873-3468.13915⟩
ISSN: 0014-5793
1873-3468
DOI: 10.1002/1873-3468.13915⟩
Popis: International audience; Staphylococcus aureus is a bacterial pathogen and one of the leading causes of healthcare-acquired infections in the world. The growing antibiotic resistance of S. aureus obliges us to search for new drugs Accepted Article This article is protected by copyright. All rights reserved and treatments. As the majority of antibiotics target the ribosome, knowledge of its detailed structure is crucial for drug development. Here, we report the Cryo-EM reconstruction at 3.2 Å resolution of the S. aureus ribosome with P-site tRNA, messenger RNA and ten RNA modification sites previously not assigned or visualized. The resulting model is the most precise and complete high-resolution structure to date of the S. aureus 70S ribosome with functional ligands.
Databáze: OpenAIRE
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