Acute Toxicity Assessment: Macroscopic and Ultrastructural Effects in Mice Treated with Oral Tetrodotoxin
| Autor: | Natalia Vilariño, Luis M. Botana, M. Carmen Louzao, Mercedes R. Vieytes, Paula Abal |
|---|---|
| Přispěvatelé: | Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Health
Toxicology and Mutagenesis lcsh:Medicine Administration Oral Pharmacology Toxicology Kidney chemistry.chemical_compound Mice Lipid droplet Ingestion Lung tetrodotoxin 0303 health sciences Food poisoning musculoskeletal neural and ocular physiology 030302 biochemistry & molecular biology Stomach Brain 3. Good health Mitochondria Intestines Liver mitochondria swelling macroscopic alterations Tetrodotoxin Female Endoplasmic Reticulum Rough Neurotoxins Biology Neurotransmission Article 03 medical and health sciences Microscopy Electron Transmission Seizures medicine Toxicity Tests Acute Animals Paralysis 14. Life underwater 030304 developmental biology gastrointestinal effect rough endoplasmic reticulum swelling Endoplasmic reticulum Myocardium lcsh:R medicine.disease Acute toxicity chemistry nervous system Ultrastructure ultrastructural changes Spleen |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Toxins Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname Toxins, Vol 11, Iss 6, p 305 (2019) Volume 11 Issue 6 |
| Popis: | Tetrodotoxin (TTX) is an extremely toxic marine compound produced by different genera of bacteria that can reach humans through ingestion mainly of pufferfish but also of other contaminated fish species, marine gastropods or bivalves. TTX blocks voltage-gated sodium channels inhibiting neurotransmission, which in severe cases triggers cardiorespiratory failure. Although TTX has been responsible for many human intoxications limited toxicological data are available. The recent expansion of TTX from Asian to European waters and diversification of TTX-bearing organisms entail an emerging risk of food poisoning. This study is focused on the acute toxicity assessment of TTX administered to mice by oral gavage following macroscopic and microscopic studies. Necropsy revealed that TTX induced stomach swelling 2 h after administration, even though no ultrastructural alterations were further detected. However, transmission electron microscopy images showed an increase of lipid droplets in hepatocytes, swollen mitochondria in spleens, and alterations of rough endoplasmic reticulum in intestines as hallmarks of the cellular damage. These findings suggested that gastrointestinal effects should be considered when evaluating human TTX poisoning. The research leading to these results has received funding from the following FEDER cofunded-grants. From CDTI and Technological Funds, supported by Ministerio de Economía, Industria y Competitividad, AGL2016-78728-R (AEI/FEDER, UE) AGL2014-58210-R, ISCIII/PI16/01830 and RTC-2016-5507-2, ITC-20161072. From Conselleria de Cultura, Educacion e Ordenación Universitaria, Xunta de Galicia, 2017 GRC GI-1682 (ED431C 2017/01). From European Union POCTEP 0161-Nanoeaters -1-E-1, Interreg AlertoxNet EAPA-317-2016, Interreg Agritox EAPA-998-2018, and H2020 778069-EMERTOX SI |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|