TRPV4 channels activity in bovine articular chondrocytes: Regulation by obesity-associated mediators
| Autor: | Robert Wilkins, Diego F. López-Zapata, Julio C. Sánchez |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Cartilage
Articular Leptin TRPV4 medicine.medical_specialty Patch-Clamp Techniques Physiology Morpholines medicine.medical_treatment Interleukin-1beta TRPV Cation Channels Adipokine Gadolinium Chondrocyte Calcium in biology Proinflammatory cytokine Chondrocytes Osmotic Pressure Internal medicine medicine Animals Insulin Pyrroles Resistin Obesity Molecular Biology Cells Cultured Tumor Necrosis Factor-alpha Chemistry Cell Biology Ruthenium Red medicine.anatomical_structure Endocrinology Calcium Cattle Mechanosensitive channels |
| Zdroj: | Cell Calcium. 56:493-503 |
| ISSN: | 0143-4160 |
| DOI: | 10.1016/j.ceca.2014.10.006 |
| Popis: | Turnover of the cartilage extracellular matrix depends exclusively on chondrocytes and varies in response to load and osmolarity fluctuations. Obesity can affect chondrocyte physiology; adipokines, insulin and proinflammatory cytokines levels are all altered in the obese and are related to matrix turnover impairments and thus to osteoarthritis. TRPV4, a mechanosensitive cation channel, is responsible for reacting to hypotonic variations. In this study, the presence and activity of TRPV4 channels in bovine chondrocytes were evaluated using the whole-cell patch-clamp technique and fluorescence measurements to perform characterisations of these channels and to determine intracellular calcium responses. The expression of TRPV4 was determined by RT-PCR. The TRPV4 regulation by hypotonic shock, insulin and adipokines were analysed. Hypoosmolarity induced a Gd(3+)-, ruthenium red-, and HC-067047-sensitive current, predominantly inward, an intracellular Ca(2+) concentration increase and a membrane depolarisation. The current had a reversal potential of +28±4mV and exhibited preferential permeability to Ca(2+); 4αPDD, a specific TRPV4 agonist, evoked the same response. TNFα, IL-1β, insulin, and, to a lesser degree, leptin and resistin attenuated the TRPV4-mediated effects; in contrast, adiponectin did not affect them. These results confirm the function of TRPV4 in bovine articular chondrocytes and its regulation by obesity-associated mediators. |
| Databáze: | OpenAIRE |
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