Methods to study differences in cell mobility during skin wound healing in vitro
| Autor: | Sanne Roffel, Amit Gefen, Ester M. Weijers, Melanie Breetveld, Mireille A. Boink, Susan Gibbs, Lenie J. van den Broek, Hanneke N. Monsuur |
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| Přispěvatelé: | Oral Cell Biology, Oral Biochemistry, Orale Celbiologie (ORM, ACTA), Orale Biochemie (OII, ACTA), Dermatology, MOVE Research Institute |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Keratinocytes
0301 basic medicine Pathology medicine.medical_specialty Biomedical Engineering Biophysics In Vitro Techniques Melanocyte Fibroblast migration 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Dermis Cell Movement Skin Physiological Phenomena medicine Humans Orthopedics and Sports Medicine Fibroblast Cells Cultured Skin Wound Healing Tissue Engineering Epidermis (botany) Chemistry Rehabilitation Endothelial Cells Cell Differentiation Fibroblasts Cell biology Endothelial stem cell 030104 developmental biology medicine.anatomical_structure Melanocytes Biological Assay Keratinocyte Wound healing |
| Zdroj: | Monsuur, H N, Boink, M A, Weijers, E M, Roffel, S, Breetveld, M, Gefen, A, van den Broek, L J & Gibbs, S 2016, ' Methods to study differences in cell mobility during skin wound healing in vitro ', Journal of Biomechanics, vol. 49, no. 8, pp. 1381-1387 . https://doi.org/10.1016/j.jbiomech.2016.01.040 Journal of Biomechanics, 49(8), 1381-1387. Elsevier Limited Journal of Biomechanics, 49(8), 1381-1387. Elsevier |
| ISSN: | 0021-9290 |
| DOI: | 10.1016/j.jbiomech.2016.01.040 |
| Popis: | Wound healing events which occur in humans are difficult to study in animals due to differences in skin physiology. Furthermore there are increasing restrictions in Europe for using animals for testing the therapeutic properties of new compounds. Therefore, in line with the 3Rs (reduction, refinement and replacement of test animals), a number of human in vitro models of different levels of complexity have been developed to investigate cell mobility during wound healing. Keratinocyte, melanocyte, fibroblast and endothelial cell mobility are described, since these are the residential cells which are responsible for restoring the main structural features of the skin. A monolayer scratch assay is used to study random fibroblast and endothelial cell migration in response to EGF and bFGF respectively and a chemotactic assay is used to study directional fibroblast migration towards CCL5. In order to study endothelial sprouting in response to bFGF or VEGF, which involves continuous degradation and resynthesis of a 3D matrix, a fibrin gel is used. Human physiologically relevant tissue-engineered skin models are used to investigate expansion of the stratified, differentiated epidermis (keratinocytes and melanocytes) over a fibroblast populated dermis and also to study migration and distribution of fibroblasts into the dermis. Together these skin models provide a platform for testing the mode of action of novel compounds for enhanced and scar free wound healing. |
| Databáze: | OpenAIRE |
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