Assessment of microbiological correlates and immunostimulatory potential of electron beam inactivated metabolically active yet non culturable (MAyNC) Salmonella Typhimurium

Autor: Palmy R. Jesudhasan, Noah D. Cohen, Sohini S. Bhatia, Robert C. Alaniz, Chandni Praveen, Suresh D. Pillai, Robert E. Droleskey
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Bacterial Diseases
CD4-Positive T-Lymphocytes
Salmonella typhimurium
Salmonella
Physiology
Cell Membranes
medicine.disease_cause
Pathology and Laboratory Medicine
White Blood Cells
Mice
Medical Conditions
Animal Cells
Immune Physiology
Medicine and Health Sciences
Immune Response
Cells
Cultured
Innate Immune System
Vaccines
Multidisciplinary
Attenuated vaccine
Chemistry
T Cells
Immunogenicity
Salmonella vaccine
Bacterial Pathogens
Infectious Diseases
Medical Microbiology
Medicine
Cytokines
Female
Pathogens
Cellular Types
Cellular Structures and Organelles
Research Article
Attenuated Vaccines
Infectious Disease Control
Salmonella Vaccines
Science
Immune Cells
030106 microbiology
Immunology
chemical and pharmacologic phenomena
Cytotoxic T cells
Electrons
Vaccines
Attenuated
Microbiology
03 medical and health sciences
Immune system
Antigen
Enterobacteriaceae
medicine
Animals
Microbial Pathogens
Salmonella Infections
Animal
Blood Cells
Bacteria
Organisms
Biology and Life Sciences
Dendritic cell
Cell Biology
Dendritic Cells
Molecular Development
In vitro
Mice
Inbred C57BL
030104 developmental biology
Vaccines
Inactivated
Immune System
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 4, p e0243417 (2021)
ISSN: 1932-6203
Popis: This study investigates the microbiological and immunological basis underlying the efficacy of electron beam-inactivated immune modulators. The underlying hypothesis is that exposure to eBeam-based ionization reactions inactivate microorganisms without modifying their antigenic properties and thereby creating immune modulators. The immunological correlates of protection induced by such eBeam based Salmonella Typhimurium (EBST) immune modulators in dendritic cell (DC) (in vitro) and mice (in vivo) models were assessed. The EBST stimulated innate pro inflammatory response (TNFα) and maturation (MHC-II, CD40, CD80 and CD86) of DC. Immuno-stimulatory potential of EBST was on par with both a commercial Salmonella vaccine, and live Salmonella cells. The EBST cells did not multiply under permissive in vitro and in vivo conditions. However, EBST cells remained metabolically active. EBST immunized mice developed Salmonella-specific CD4+ T-cells that produced the Th1 cytokine IFNγ at a level similar to that induced by the live attenuated vaccine (AroA- ST) formulation. The EBST retained stable immunogenic properties for several months at room temperature, 4°C, and -20°C as well as after lyophilization. Therefore, such eBeam-based immune modulators have potential as vaccine candidates since they offer the safety of a “killed” vaccine, while retaining the immunogenicity of an “attenuated” vaccine. The ability to store eBeam based immune modulators at room temperature without loss of potency is also noteworthy.
Databáze: OpenAIRE
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