The Na+/H+ antiporter of the thermohalophilic bacterium Rhodothermus marinus
| Autor: | Nuno A.M. Felix, Ana M.P. Melo, Miguel Teixeira, João N. Carita, Lígia M. Saraiva |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Sodium-Hydrogen Exchangers
Transcription Genetic Operon Protein subunit Antiporter Molecular Sequence Data Biophysics Rhodothermus Biology medicine.disease_cause Quinone oxidoreductase Biochemistry chemistry.chemical_compound medicine Amino Acid Sequence Pterin Molecular Biology Escherichia coli Electron Transport Complex I Escherichia coli Proteins Gene Expression Regulation Bacterial Cell Biology Hydrogen-Ion Concentration Antiporters chemistry Dehydratase |
| Zdroj: | Biochemical and Biophysical Research Communications. 348:1011-1017 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2006.07.134 |
| Popis: | In the thermohalophilic bacterium Rhodothermus marinus, the NADH:quinone oxidoreductase (complex I) is encoded by two single genes and two operons, one of which contains the genes for five complex I subunits, nqo10–nqo14, a pterin carbinolamine dehydratase, and a putative single subunit Na+/H+ antiporter. Here we report that the latter encodes indeed a functional Na+/H+ antiporter, which is able to confer resistance to Na+, but not to Li+ to an Escherichia coli strain defective in Na+/H+ antiporters. In addition, an extensive amino acid sequence comparison with several single subunit Na+/H+ antiporters from different groups, namely NhaA, NhaB, NhaC, and NhaD, suggests that this might be the first member of a new type of Na+/H+ antiporters, which we propose to call NhaE. |
| Databáze: | OpenAIRE |
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