The role of Bacillus acidophilus in osteoporosis and its roles in proliferation and differentiation

Autor: Yuming Wang, Qiang Zhang, Shuzhi Feng, Baokang Dong, Chen Chen, Yu Zhu, Bangmao Wang
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Microbiology (medical)
medicine.medical_specialty
Clinical Biochemistry
Osteoclasts
Bacillus
Gut flora
Cell Line
03 medical and health sciences
Mice
0302 clinical medicine
Lactobacillus acidophilus
Lactobacillus rhamnosus
Lactobacillus
Internal medicine
medicine
Cathepsin K
Immunology and Allergy
Animals
Humans
Vitamin D
Research Articles
Cell Proliferation
Aged
80 and over
Osteoblasts
biology
gut microbiota
Lacticaseibacillus rhamnosus
Biochemistry (medical)
Public Health
Environmental and Occupational Health
food and beverages
Cell Differentiation
Hematology
biology.organism_classification
osteoporosis
Peptide Fragments
Gastrointestinal Microbiome
RUNX2
Medical Laboratory Technology
030104 developmental biology
Endocrinology
RAW 264.7 Cells
030220 oncology & carcinogenesis
Osteocalcin
biology.protein
Alkaline phosphatase
Procollagen
Research Article
Zdroj: Journal of Clinical Laboratory Analysis
ISSN: 1098-2825
0887-8013
Popis: Background Osteoporosis is one of the most closely related diseases associated with the elderly. In recent years, the studies found that gut microbiota can cause osteoporosis. We evaluated the role of Bacillus acidophilus in osteoporosis and its roles in proliferation and differentiation. Methods We selected 5 healthy people and 10 osteoporosis patients and analyzed their level of 25‐hydroxyvitamin D and procollagen type I N‐terminal peptide (PINP), the characteristic of gut microbiota. The effect of lactobacillus acidophilus and Lactobacillus rhamnosus supernatant and butanoic acids on proliferation, differentiation, and maturity of osteoblasts MC3T3‐E1 and osteoclasts RAW 264.7 cells and the activity of alkaline phosphatase, concentration of osteocalcin, and the expression of RUNX2, RANK, NFATc1, cathepsin K, DC‐STAMP, OSCAR, WNT2, and CTNNB1 were measured in the above cell lines. Results The diversity of gut microbiota in osteoporosis patients is decreased and imbalanced with lower abundance of lactobacillus and butyric acid bacteria; meanwhile, 25‐hydroxyvitamin D and PINP of osteoporosis patient were significantly lower than the normal group. The proliferation, differentiation, and maturity of MC3T3‐E1 cells were stimulated; the activity of alkaline phosphatase, concentration of osteocalcin, and the expression of RUNX2, NFATc1, cathepsin K, DC‐STAMP, OSCAR, WNT2, and CTNNB1 were improved by supernatant of lactobacillus acidophilus, Lactobacillus rhamnosus and butanoic acids; however, the proliferation, differentiation, maturity, and the expression of RANK, NFATc1, cathepsin K, DC‐STAMP, OSCAR, WNT2, and CTNNB1 in RAW 264.7 cells were suppressed. Conclusions The lactobacillus acidophilus and Lactobacillus rhamnosus supernatant could stimulate the proliferation, differentiation, and maturation of osteoblasts; the production of butyric acid may be the potential mechanism.
The diversity of gut microbiota is decreased and imbalance with lower abundance of lactobacillus and butyric acid bacteria in osteoporosis patients. The gut microbiota regulate the proliferation, differentiation, and maturation of osteoblasts, and the production of butyric acid may be the potential mechanism.
Databáze: OpenAIRE
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