Dietary supplement for energy and reduced appetite containing the β-agonist isopropyloctopamine leads to heart problems and hospitalisations
| Autor: | Hans G.J. Mol, Toine F H Bovee, Gerard D van Bruchem, Ron L.A.P. Hoogenboom, H.H. Heskamp, Martin Kooijman, Monique Bienenmann-Ploum, Ruud van Dam, J.J.P. Lasaroms, Leendert A. van Ginkel |
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| Rok vydání: | 2016 |
| Předmět: |
Novel Foods & Agrochains
Health Toxicology and Mutagenesis health risks enforcement Biosensing Techniques 02 engineering and technology Pharmacology Novel Foods & Agroketens Toxicology 01 natural sciences BU Veterinary Drugs Foodborne Diseases supplements BU Contaminants & Toxins Anabolic Agents Food Labeling web shops Pausinystalia Radioligand Medicine Bioassay Multiplex BU Toxicology Novel Foods & Agrochains Directie Nootropic Agents Netherlands BU Toxicology General Medicine 021001 nanoscience & nanotechnology Hospitalization BU Toxicologie Novel Foods & Agroketens Performance-Enhancing Substances 0210 nano-technology Rikilt B&T Toxicologie en Effectanalyse Agonist Heart Diseases BU Toxicologie medicine.drug_class BU Contaminanten & Toxines Adrenergic beta-3 Receptor Agonists Food Contamination BU Dierbehandelingsmiddelen Alkaloids Therapeutic index Appetite Depressants Humans VLAG business.industry 010401 analytical chemistry Public Health Environmental and Occupational Health General Chemistry Food Inspection 0104 chemical sciences Dietary Supplements Plant Preparations internet business Quantitative analysis (chemistry) Antipyrine Biosensor Food Science Food contaminant |
| Zdroj: | Food Additives & Contaminants. Pt. A, Chemistry, Analysis, Control, Exposure & Risk Assessment, 33(5), 749-759 Food Additives & Contaminants. Pt. A, Chemistry, Analysis, Control, Exposure & Risk Assessment 33 (2016) 5 |
| ISSN: | 1944-0057 1944-0049 |
| DOI: | 10.1080/19440049.2016.1167965 |
| Popis: | In 2013 the Dutch authorities issued a warning against a dietary supplement that was linked to 11 reported adverse reactions, including heart problems and in one case even a cardiac arrest. In the UK a 20-year-old woman, said to have overdosed on this supplement, died. Since according to the label the product was a herbal mixture, initial LC-MS/MS analysis focused on the detection of plant toxins. Yohimbe alkaloids, which are not allowed to be present in herbal preparations according to Dutch legislation, were found at relatively high levels (400-900 mg kg(-1)). However, their presence did not explain the adverse health effects reported. Based on these effects the supplement was screened for the presence of a β-agonist, using three different biosensor assays, i.e. the validated competitive radioligand β2-adrenergic receptor binding assay, a validated β-agonists ELISA and a newly developed multiplex microsphere (bead)-based β-agonist assay with imaging detection (MAGPIX(®)). The high responses obtained in these three biosensors suggested strongly the presence of a β-agonist. Inspection of the label indicated the presence of N-isopropyloctopamine. A pure standard of this compound was bought and shown to have a strong activity in the three biosensor assays. Analysis by LC-full-scan high-resolution MS confirmed the presence of this 'unknown known' β3-agonist N-isopropyloctopamine, reported to lead to heart problems at high doses. A confirmatory quantitative analysis revealed that one dose of the preparation resulted in an intake of 40-60 mg, which is within the therapeutic range of this compound. The case shows the strength of combining bioassays with chemical analytical techniques for identification of illegal pharmacologically active substances in food supplements. |
| Databáze: | OpenAIRE |
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