c-K-ras codon 12 GGT-CGT point mutation. An infrequent event in human lung cancer
| Autor: | Antonio Milici, Edwin C. Murphy, Mark Blick, Jordan U. Gutterman |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Genetics
Silent mutation Mutation rate Lung Neoplasms Point mutation Nonsense mutation Biophysics Cell Biology DNA Restriction Enzymes Oncogenes Biology Biochemistry Molecular biology Cell Transformation Neoplastic Mutation (genetic algorithm) Mutation Missense mutation Humans RNA Messenger Restriction fragment length polymorphism Restriction Enzyme Cleavage Site Codon Molecular Biology Polymorphism Restriction Fragment Length |
| Zdroj: | Biochemical and biophysical research communications. 140(2) |
| ISSN: | 0006-291X |
| Popis: | Hu-c-ras represent a family of oncogenes which are capable of inducing malignant transformation in the NIH/3T3 mouse cell line. Associated with this transformation are specific point mutations observed in the 12th and 61st codon of c-K-ras and N-ras and c-Ha-ras, respectively. These base changes generate, in some instances, a new restriction enzyme cleavage site and a restriction fragment length polymorphism (RFLP). One such RFLP has recently been reported for the mutation GGT-CGT at codon 12 of c-K-ras. Our data suggest that this point mutation is rarely present in human lung cancer and therefore is not likely to play a major role in cancer development. |
| Databáze: | OpenAIRE |
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