Antimicrobial activity of a UV-stable bacteriocin-like inhibitory substance (BLIS) produced by Enterococcus faecium strain DSH20 against vancomycin-resistant Enterococcus (VRE) strains
| Autor: | Hossein Fazeli, Fatemeh Khodabakhsh, Sina Mobasherizadeh, Dariush Shokri, Saeideh Zaghian, Behrooz Ataei |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Microbiology (medical)
Methicillin-Resistant Staphylococcus aureus Klebsiella Enterococcus faecium Biology medicine.disease_cause BLIS Enterococcus faecalis Microbiology Vancomycin-Resistant Enterococci Bacteriocin Bacteriocins Drug Stability Immunology and Microbiology(all) Drug Resistance Multiple Bacterial Gram-Negative Bacteria medicine Immunology and Allergy Vancomycin-resistant Enterococcus Antibacterial agent General Immunology and Microbiology Vancomycin-resistant Enterococcus (VRE) Temperature General Medicine Hydrogen-Ion Concentration biochemical phenomena metabolism and nutrition Antimicrobial biology.organism_classification Pandrug resistant (PDR) Infectious Diseases Enterococcus |
| Zdroj: | Journal of Microbiology, Immunology and Infection. (5):371-376 |
| ISSN: | 1684-1182 |
| DOI: | 10.1016/j.jmii.2013.05.004 |
| Popis: | Background/purpose The narrow spectrum of action of most bacteriocins is an important limitation for their application as antimicrobial agents. The current study describes a novel bacteriocin-like inhibitory substance (BLIS) that display extended spectrum antimicrobial activity against vancomycin-resistant Enterococcus (VRE) strains. Methods Acquired resistance profiles of Enterococcus isolates determined based on the European Centre for Disease Prevention and Control (ECDC) and the Centers for Disease Control and Prevention (CDC) definition as multidrug-resistant (MDR), extensively drug-resistant (XDR) and pandrug resistant (PDR). BLIS activity of Enterococcus isolates was investigated against Enterococcus faecalis ( E. faecalis ) ATCC 29212 as the indicator strain and clinical isolates including VRE, methicillin resistant Staphylococcus aureus (MRSA) and Gram-negative bacteria containing Pseudomonas aeruginosa ( P. aeruginosa ), Klebsiella , Acinetobacter , and Escherichia coli ( E. coli ). Results Among 273 Enterococcus isolates, 27 and 2 VRE isolates, respectively, were XDR and PDR and eight isolates had BLIS activity against the indicator strain. One of these isolates, identified as E. faecium strain DSH20 based on its phenotypical and biochemical properties, as well as its 16S rRNA gene sequence, had potent BLIS production against all 29 VRE strains, but had no activity against MRSA, P. aeruginosa , Klebsiella , Acinetobacter , and E. coli strains. It was heat stable up to 121°C for 15 minutes (autoclave condition), active within the pH range of 3–9 and had UV stability, but its activity disappeared by treatment with proteinase K, pepsin, and trypsin, demonstrating its proteinaceous nature. It was designated as an approximately 35kDa peptide using the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method. Conclusion This peptide is a potential agent for use as an alternative antibacterial agent for the treatment of drug-resistant strains of VRE infection. |
| Databáze: | OpenAIRE |
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