Intracellular Localization of a Class IV Chitinase from Yam
| Autor: | Dai Yuki, Daizo Koga, Takuji Mitsunaga, Minoru Iwase |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
DNA
Plant Nicotiana tabacum Dioscoreaceae Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Transformation Genetic alpha-Mannosidase Tobacco Molecular Biology Gene Cellular localization biology Dioscorea Chitinases fungi Organic Chemistry food and beverages General Medicine Plants Genetically Modified biology.organism_classification Dioscorea oppositifolia genomic DNA Vacuoles Chitinase biology.protein Rhizobium Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 68:1518-1524 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.68.1518 |
| Popis: | Genomic DNA encoding a class IV chitinase was cloned from yam (Dioscorea opposita Thunb) leaves in previous research (Biosci. Biotechnol. Biochem., 68, 1508-1517 (2004)). But this chitinase had an additional sequence composed of eight amino acids (a C-terminal extension) at the C-terminal, compared with class IV chitianses from other plants. In order to clarify the role of this C-terminal extension in cellular localization, plants and suspension-cultured cells of Nicotiana tabacum were transformed with either the cloned yam class IV chitinase gene carrying the C-terminal extension or its truncated gene by the Agrobacterium-mediated method, and then their localization was investigated. The results suggest that the C-terminal extension of yam class IV chitinase plays a role as a targeting signal for plant vacuoles. This is the first report presenting the existence of vacuolar type class IV chitinase. |
| Databáze: | OpenAIRE |
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