Identification of flavonoids and flavonoid rhamnosides from Rhododendron mucronulatum for. albiflorum and their inhibitory activities against aldose reductase
| Autor: | So-Youn Mok, Sanghyun Lee |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Rhododendron
Flavonoid Flowers Rhododendron mucronulatum Analytical Chemistry Rats Sprague-Dawley chemistry.chemical_compound Aldehyde Reductase Lens Crystalline Animals Glycosides Afzelin Enzyme Inhibitors Flavonoids chemistry.chemical_classification Chromatography biology Plant Extracts Chemistry General Medicine biology.organism_classification Quercitrin Rats Kinetics Biochemistry Myricetin Kaempferol Myricitrin Quercetin Food Science |
| Zdroj: | Food Chemistry. 136:969-974 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2012.08.091 |
| Popis: | To investigate the therapeutic potential of compounds from natural sources, Rhododendron mucronulatum for. albiflorum flowers (RMAF) and R. mucronulatum flowers (RMF) were tested for inhibition of aldose reductase (AR). The methanol extracts of RMAF and RMF exhibited AR inhibitory activities (IC(50) values 1.07 and 1.29 μg/mL, respectively). The stepwise polarity fractions of RMAF were tested for in vitro inhibition of AR from rat lenses. Of these, the ethyl acetate (EtOAc) fraction exhibited AR inhibitory activity (IC(50) 0.15 μg/mL). A chromatography of the active EtOAc fraction of RMAF led to the isolation of six flavonoids, which were identified by spectroscopic analysis as kaempferol (1), afzelin (2), quercetin (3), quercitrin (4), myricetin (5) and myricitrin (6). Compounds 1-6 exhibited high AR inhibitory activity, with IC(50) values of 0.79, 0.31, 0.48, 0.13, 11.92 and 2.67 μg/mL, respectively. HPLC/UV analysis revealed that the major flavonoids of RMAF and RMF are quercitrin (4) and myricitrin (6). Our results suggest that RMAF containing these six flavonoids could be a useful natural source in the development of a novel AR inhibitory agent against diabetic complications. |
| Databáze: | OpenAIRE |
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