Inhibitory effects of polyoxyethylene stearate, PANTA, and neutral pH on growth of Mycobacterium genavense in BACTEC primary cultures

Autor: Françoise Portaels, Laurence Realini, P. Van Der Stuyft, Bernard Hirschel, K. De Ridder
Rok vydání: 1997
Předmět:
Microbiology (medical)
Bacilli
Surface-Active Agents/ pharmacology
Mycobacterium genavense
Mice
Nude
Microbial Sensitivity Tests
Polyethylene Glycols/ pharmacology
Vial
Microbiology
Mycobacterium
Polyethylene Glycols
Mycobacterium/drug effects/ growth & development/isolation & purification
chemistry.chemical_compound
Mice
Surface-Active Agents
Stearate
Animals
Humans
Neutral ph
ddc:616
Aids patients
Bacteriological Techniques
Mycobacterium Infections
biology
AIDS-Related Opportunistic Infections
Hydrogen-Ion Concentration
biology.organism_classification
United States
Anti-Bacterial Agents
Culture Media
AIDS-Related Opportunistic Infections/ microbiology
Drug Therapy
Combination/ pharmacology
chemistry
Mycobacterium Infections/ microbiology
Drug Therapy
Combination
Bacteria
Switzerland
Research Article
Zdroj: Journal of Clinical Microbiology, Vol. 35, No 11 (1997) pp. 2791-2794
ISSN: 0095-1137
Popis: We report on the influences of polyoxyethylene stearate (POES), PANTA, and pH on primary cultures of Mycobacterium genavense in BACTEC vials. As a model for primary cultures from tissue, seven different strains first isolated from AIDS patients (five from Switzerland and two from the United States) were inoculated into nude mice in order to obtain large amounts of bacilli to test different conditions simultaneously. Our results demonstrate that the size of the inoculum (10[6] acid-fast bacilli/vial), an acid pH (pH 6.0), and the absence of additives (POES and PANTA) significantly (P < 0.001) increased the probability of a successful culture in 1 month, considering growth index (GI) of > or =100 or a GI of > or =999 as criterion of success. In logistic regression analysis, all factors maintained a significant (P < 0.001) independent effect, and no interactions were observed between them. The best conditions for the primary cultures of M. genavense were the use of Middlebrook 7H12 medium at pH 6.0 without any additives.
Databáze: OpenAIRE
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