An Evolutionary Cancer Epigenetic Approach Revealed DNA Hypermethylation of Ultra-Conserved Non-Coding Elements in Squamous Cell Carcinoma of Different Mammalian Species
| Autor: | Marco Morandi, Silvia Sabattini, Eva Dervas, Alexandria Schauer, Achille Tarsitano, Antonella Rigillo, Andrea Renzi, Caterina Tonon, Luca Morandi, Giuliano Bettini, Davide B Gissi, Stefania Evangelisti |
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| Přispěvatelé: | Morandi L., Sabattini S., Renzi A., Rigillo A., Bettini G., Dervas E., Schauer A., Morandi M., Gissi D.B., Tarsitano A., Evangelisti S., Tonon C. |
| Rok vydání: | 2020 |
| Předmět: |
Male
squamous cell carcinoma endocrine system Bisulfite sequencing evolutionary epigenetic Biology Article DNA sequencing Epigenesis Genetic Evolution Molecular Dogs Mustelidae otorhinolaryngologic diseases medicine Animals Humans Horses Prospective Studies Epigenetics lcsh:QH301-705.5 Stomatitis Conserved Sequence Aged Retrospective Studies DNA methylation CATS ultra-conserved non-coding elements technology industry and agriculture Cancer General Medicine Porcupines medicine.disease Molecular biology Bisulfite lcsh:Biology (General) Carcinoma Squamous Cell Cats bisulfite sequencing Cattle CpG Islands Female Mouth Neoplasms evolutionary epigenetics |
| Zdroj: | Cells Volume 9 Issue 9 Cells, Vol 9, Iss 2092, p 2092 (2020) |
| ISSN: | 2073-4409 |
| DOI: | 10.3390/cells9092092 |
| Popis: | Background: Ultra-conserved non-coding elements (UCNEs) are genomic sequences that exhibit > 95% sequence identity between humans, mammals, birds, reptiles, and fish. Recent findings reported their functional role in cancer. The aim of this study was to evaluate the DNA methylation modifications of UNCEs in squamous cell carcinoma (SCC) from different mammal species. Methods: Fifty SCCs from 26 humans, 17 cats, 3 dogs, 1 horse, 1 bovine, 1 badger, and 1 porcupine were investigated. Fourteen feline stomatitis and normal samples from 36 healthy human donors, 7 cats, 5 dogs, 5 horses, 2 bovines and 1 badger were collected as normal controls. Bisulfite next generation sequencing evaluated the DNA methylation level from seven UCNEs (uc.160, uc.283, uc.416, uc.339, uc.270, uc.299, and uc.328). Results: 57/59 CpGs were significantly different according to the Kruskal&ndash Wallis test (p < 0.05) comparing normal samples with SCC. A common DNA hypermethylation pattern was observed in SCCs from all the species evaluated in this study, with an increasing trend of hypermethylation starting from normal mucosa, through stomatitis to SCC. Conclusions: Our findings indicate that UCNEs are hypermethylated in human SCC, and this behavior is also conserved among different species of mammals. |
| Databáze: | OpenAIRE |
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