Expression of the nitrate transporter nrt2 gene from the symbiotic basidiomycete Hebeloma cylindrosporum is affected by host plant and carbon sources

Autor: Roland Marmeisse, Régis Pépin, Marie-Christine Verner, Laurence Fraissinet-Tachet, Jean-Claude Debaud, David Rekangalt
Přispěvatelé: Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2009
Předmět:
0106 biological sciences
Nitrogen
Anion Transport Proteins
Plant Science
Biology
01 natural sciences
Fungal Proteins
03 medical and health sciences
chemistry.chemical_compound
Nitrate
Gene Expression Regulation
Fungal
Botany
Genetics
Hebeloma
Ammonium
Sugar
Molecular Biology
Ecology
Evolution
Behavior and Systematics
Mycelium
[ SDV.MP.MYC ] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
030304 developmental biology
Regulation of gene expression
0303 health sciences
[ SDE.BE ] Environmental Sciences/Biodiversity and Ecology
Host (biology)
fungi
Fructose
Nitrate Transporters
General Medicine
Pinus
Carbon
Nitrate uptake . Nitrate transporter . Nitrogen and carbon regulation . Ectomycorrhiza . Hebeloma cylindrosporum
Ectomycorrhiza
chemistry
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
010606 plant biology & botany
Zdroj: Mycorrhiza
Mycorrhiza, Springer Verlag, 2009, pp.143-148. ⟨10.1007/s00572-008-0221-2⟩
Mycorrhiza, Springer Verlag, 2009, pp.143-148. 〈10.1007/s00572-008-0221-2〉
ISSN: 0940-6360
1432-1890
DOI: 10.1007/s00572-008-0221-2⟩
Popis: International audience; Although the function of the extramatrical mycelium of ectomycorrhizal fungi is considered essential for the acquisition of nitrogen by forest trees, gene regulation in this fungal compartment is poorly characterized. In this study, the expression of the nitrate transporter gene nrt2 from the ectomycorrhizal basidiomycete Hebeloma cylindrosporum was shown to be regulated by plant host and carbon sources. In the presence of a low fructose concentration, nrt2 expression could not be detected in the free-living mycelium but was high in the extramatrical symbiotic mycelium associated to the host plant Pinus pinaster. In the absence of nitrogen or in the presence of nitrate, high sugar concentrations in the medium were able to enhance nrt2 expression. Nevertheless, in the presence of high fructose concentration, high ammonium concentration still completely repressed nrt2 expression indicating that the nitrogen repression overrides sugar stimulation. This is the first report revealing an effect of host plant and of carbon sources on the expression of a fungal nitrate transporter-encoding gene
Databáze: OpenAIRE
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