Transcription profile in mouse four-cell, morula, and blastocyst: Genes implicated in compaction and blastocoel formation
| Autor: | Yong-Ju Bae, Xiang-Shun Cui, Nam-Hyung Kim, Xing-Yu Li, Jason-Jongho Kang, Xing-Hui Shen |
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| Rok vydání: | 2006 |
| Předmět: |
Transcription
Genetic Cell Embryonic Development Biology Morula Mice Gene expression Genetics medicine Animals Blastocyst Gene DNA Primers Oligonucleotide Array Sequence Analysis Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Embryogenesis Blastocoel Gene Amplification Reproducibility of Results Embryo Cell Biology Cell biology medicine.anatomical_structure Gene chip analysis RNA Developmental Biology |
| Zdroj: | Molecular Reproduction and Development. 74:133-143 |
| ISSN: | 1098-2795 1040-452X |
| DOI: | 10.1002/mrd.20483 |
| Popis: | To gain insight into early embryo development, we utilized microarray technology to compare gene expression profiles in four-cell (4C), morula (MO), and blastocyst (BL) stage embryos. Differences in spot intensities were normalized, and grouped by using Avadis Prophetic software platform (version 3.3, Strand Genomics Ltd.) and categories were based on the PANTHER and gene ontology (GO) classification system. This technique identified 622 of 7,927 genes as being more highly expressed in MO when compared to 4C (P |
| Databáze: | OpenAIRE |
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