Immunohistochemistry is not an accurate first step towards the molecular diagnosis of MUTYH-associated polyposis
| Autor: | Rachel S. van der Post, Marjolijn J. L. Ligtenberg, Carolien M. Kets, Nicoline Hoogerbrugge, Joannes H. J. M. van Krieken |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adenoma
Adult Male Cytoplasm Pathology medicine.medical_specialty Age-related aspects of cancer [ONCOL 2] Genetics and epigenetic pathways of disease [NCMLS 6] Biology medicine.disease_cause Germline DNA Glycosylases Pathology and Forensic Medicine Diagnosis Differential Molecular epidemiology [NCEBP 1] MUTYH Translational research [ONCOL 3] medicine Humans Genetic Testing Molecular Biology Germ-Line Mutation Aged Mutation Hereditary cancer and cancer-related syndromes [ONCOL 1] Intestinal Polyposis MUTYH-Associated Polyposis Antibodies Monoclonal Reproducibility of Results Cancer Cell Biology General Medicine Middle Aged medicine.disease Immunohistochemistry Monoclonal Disease Progression Mutation testing Cancer research Female Colorectal Neoplasms |
| Zdroj: | Virchows Archiv, 454, 1, pp. 25-9 Virchows Archiv, 454, 25-9 |
| ISSN: | 0945-6317 |
| Popis: | Contains fulltext : 80376.pdf (Publisher’s version ) (Open Access) Identifying patients with germline MUTYH mutation-associated polyposis is presently difficult. The aim of this study is to investigate the possibilities of IHC as a screening test to select patients for MUTYH mutation analysis. The expression of MUTYH protein in colorectal adenomas or cancer was studied by IHC using three different (1 polyclonal and 2 monoclonal) antibodies in six samples from patients with biallelic MUTYH mutations, in three samples from patients with a single MUTYH mutation, and in 11 samples from patients without MUTYH mutations. With the polyclonal antibody, adenomas and carcinomas from patients with biallelic MUTYH mutations showed a strong supranuclear cytoplasmic staining without epithelial nuclear staining. The strong supranuclear staining was also observed in the three samples from patients with a single MUTYH mutation and in nine out of 11 samples from patients without MUTYH mutations, with or without nuclear staining. Samples incubated with the monoclonal antibodies showed a non-specific pattern. Our results demonstrate that, in contrast with previous data, the cytoplasmic staining in neoplastic cells does not discriminate MUTYH mutated from unmutated cases. At present, IHC cannot be used in clinical practice to differentiate between colorectal tissue with and without germline MUTYH mutations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink