Transcriptional regulation of prostaglandin-H-synthase-1 in the amnion-derived AV3 cell line
| Autor: | K.W. Marvin, S. Potter, R.L. Eykholt, Murray D. Mitchell |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
medicine.medical_specialty Transcription Genetic Physiology medicine.medical_treatment Prostaglandin Biology Biochemistry Gene Expression Regulation Enzymologic Cell Line Proinflammatory cytokine chemistry.chemical_compound Internal medicine medicine Humans Amnion Promoter Regions Genetic Pharmacology Messenger RNA Growth factor Membrane Proteins Cell Biology beta-Galactosidase Molecular biology Isoenzymes Endocrinology medicine.anatomical_structure chemistry Prostaglandin-Endoperoxide Synthases Cell culture Cyclooxygenase 1 Arachidonic acid Tumor necrosis factor alpha |
| Zdroj: | ResearcherID |
| ISSN: | 1098-8823 |
| DOI: | 10.1016/s0090-6980(00)00102-7 |
| Popis: | Prostaglandin-H-synthase-1 (PGHS-1), while constitutively expressed in most tissues, increases in abundance in human gestational membranes at term. This suggests that PGHS-1 may be up-regulated in preparation for labor, and thus might be a key determinant in timing labor onset. We conducted transient transfection experiments in amnion-derived AV3 cells utilizing pPGHS1CAT to identify substances that might regulate PGHS-1 expression in amnion. Transforming growth factor-beta (1 ng/ml) and 15-deoxy-Delta (12,14) prostaglandin J(2) (1 muM) significantly (P < 0.05) (33% and 44% respectively) increased PGHS-1 promoter activity. The activity decreased significantly (P < 0.05) in response to interleukin-1 (IL-I)beta (1 ng/ml) (45%), tumor necrosis factor (TNF)-alpha (50 ng/ml) (34%), epidermal,growth factor (10 ng/ml) (54%), phorbol myristate acetate (10 nM) (70%), IL-4 (10 ng/ml) (50%), IL-8 (100 ng/ml) (72%) and Activin A (25 ng/ml) (32%). Whether this degree of change in promoter activity leads to physiologically relevant alterations in the amounts of PGHS-1 present in cells remains to be determined. (C) 2001 Elsevier Science Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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