Analysis of Oligosaccharides by Microbore High-Performance Liquid Chromatography
| Autor: | Joanne Charlwood, Patrick Camilleri, Helen Birrell, Jim Langridge, Edouard S. P. Bouvier |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
chemistry.chemical_classification
Glycan Chromatography biology Chemistry Molecular Sequence Data Protein primary structure Oligosaccharides Oligosaccharide Mass spectrometry Fluorescence High-performance liquid chromatography Mass Spectrometry Analytical Chemistry law.invention carbohydrates (lipids) Spectrometry Fluorescence Carbohydrate Sequence law Recombinant DNA biology.protein Glycoprotein Chromatography High Pressure Liquid |
| Zdroj: | Analytical Chemistry. 72:1469-1474 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/ac991267n |
| Popis: | A 1-mm microbore hydrophilic interaction column has been used for the separation of 2-aminoacridone (2-AMAC)-derivatized glycan mixtures, released from naturally occurring and recombinant proteins. Primary structure identification of the 2-AMAC glycan derivatives was carried out by HPLC using fluorescence and mass spectrometric detection. In some cases, enzymatic digestion of the 2-AMAC glycans was applied to confirm glycan structure. This strategy is considerably more rapid than methods normally used in glycan analysis, which involves manual collection of separated oligosaccharide derivatives and analysis of individual fractions by mass spectrometry. |
| Databáze: | OpenAIRE |
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