Synthesis, hydrolysis and stability of psilocin glucuronide
| Autor: | Heidi Pfeiffer, Jennifer Schürenkamp, Matthias Lehr, Rafaela Martin, H. Köhler |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
genetic structures
Metabolite macromolecular substances Urine Specimen Handling Pathology and Forensic Medicine Hydrolysis chemistry.chemical_compound Glucuronides Drug Stability Freezing medicine Humans Glucuronidase Whole blood chemistry.chemical_classification Chromatography Molecular Structure biology Chemistry fungi Helix pomatia Ascorbic acid biology.organism_classification Psilocybin Enzyme Biochemistry Psilocin Hallucinogens Law medicine.drug |
| Zdroj: | Forensic Science International. 237:1-6 |
| ISSN: | 0379-0738 |
| DOI: | 10.1016/j.forsciint.2014.01.006 |
| Popis: | A two-step synthesis of psilocin glucuronide (PCG), the main metabolite of psilocin, with methyl 2,3,4-tri-O-isobutyryl-1-O-trichloroacetimidoyl-α-d-glucopyranuronate is reported. With the synthesized PCG, hydrolysis conditions in serum and urine were optimized. Escherichia coli proved to be a better enzyme source for β-glucuronidase than Helix pomatia. It was essential to add ascorbic acid to serum samples to protect psilocin during incubation. Furthermore the stability of PCG and psilocin was compared as stability data are the basis for forensic interpretation of measurements. PCG showed a greater long-term stability after six months in deep frozen serum and urine samples than psilocin. The short-term stability of PCG for one week in whole blood at room temperature and in deep frozen samples was also better than that of psilocin. Therefore, PCG can be considered to be more stable than the labile psilocin and should always be included if psilocin is analyzed in samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect