First records of Geodia demosponges from the New England seamounts, an opportunity to test the use of DNA mini-barcodes on museum specimens
| Autor: | Jon A. Moore, Paco Cárdenas |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Geodiidae Geodia barretti Seamount Zoology Aquatic Science Oceanography 010603 evolutionary biology 01 natural sciences Zoologi Bathyal zone Genus SponGES Museum specimens Genetics Geodia DNA barcoding 14. Life underwater Genetik Ecology Evolution Behavior and Systematics geography Horizon 2020 geography.geographical_feature_category biology Ecology 010604 marine biology & hydrobiology Deep-sea Sponge Grounds Ecosystems of the North Atlantic: an integrated approach towards their preservation and sustainable exploitation Cytochrome c oxidase subunit I DNA degraded Holotype Grant Agreement No 679849 biology.organism_classification Porifera Mini-barcodes European Union (EU) |
| Zdroj: | Marine Biodiversity |
| Popis: | We report the first records of the sponge genus Geodia (Demospongiae, Tetractinellida, Geodiidae) from the New England Seamounts and Muir Seamount, at lower bathyal depths. Nine specimens collected between 2000 and 2005 belong to two boreal species (Geodia macandrewii and Geodia barretti) and a temperate species (Geodia megastrella). These records extend the distributions of these deep-sea amphi-Atlantic species to the west. Most of these specimens were originally fixed in formalin, which substantially degraded the DNA. We nonetheless managed to sequence two cytochrome c oxidase subunit I (COI) mini-barcodes: the universal mini-barcode at the 5′ end of the Folmer barcode (130 bp) and a newly proposed mini-barcode at the 3′ end of the Folmer barcode (296 bp). These mini-barcodes unambiguously confirmed our identifications. As an additional test, we also successfully sequenced these two mini-barcodes from the holotype of G. barretti, collected in 1855. We conclude by advocating the use of mini-barcodes on formalin-fixed or old specimens with degraded DNA. |
| Databáze: | OpenAIRE |
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