HPLC assay for 2-(3-aminopropylamino)ethanethiol (WR-1065) in plasma
| Autor: | Edward P. McGovern, Patricia D. Steele, Nollie F. Swynnerton, Donald J. Mangold |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
chemistry.chemical_classification
Male Cancer Research Analyte Radiation Chromatography Ethanethiol business.industry Coefficient of variation Radiation-Protective Agents Plasma Beagle High-performance liquid chromatography Mercaptoethylamines chemistry.chemical_compound Dogs Oncology chemistry Blood plasma Thiol Medicine Animals Radiology Nuclear Medicine and imaging business Chromatography High Pressure Liquid |
| Zdroj: | International journal of radiation oncology, biology, physics. 10(9) |
| ISSN: | 0360-3016 |
| Popis: | A high pressure liquid chromatography (HPLC) plasma assay for WR-1065 is described which is both precise (coefficient of variation less than 5%) and accurate (% average deviation less than or equal to 6.1) throughout the concentration range from 1 to 500 micrograms/mL of plasma. The analyte is separated by HPLC and detected with a thiol specific electrochemical transducer cell. The detector response is linear over the ranges 1 to 10 micrograms/mL (R2 = 0.995), 10 to 100 micrograms/mL (R2 = 0.995), and 100 to 500 micrograms/mL (R2 = 0.974). The absolute retention times for WR-1065 and WR-1729 are 9 and 12 minutes, respectively. The assay uses 100 microL of plasma and requires a total chromatography cycle time of 40 minutes. The method has been found suitable for the determination of WR-1065 in plasma from a beagle dog after i.v. administration of S-2-(3-aminopropylamino)ethyl phosphorothioate (WR-2721). |
| Databáze: | OpenAIRE |
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