The proteome pattern cGvHD_MS14 allows early and accurate prediction of chronic GvHD after allogeneic stem cell transplantation
| Autor: | Hans-Heinrich Kreipe, Jochen Metzger, Helmut Diedrich, Lothar Hambach, Hildegard T. Greinix, Christin Human, William Mullen, Anne M. Dickinson, Matthias Schiemann, Patrick Schweier, Jürgen Krauter, A Durban, D Wolf, Arnold Ganser, Gernot Beutel, Irina Türüchanow, O Böhm, Christian Könecke, Julia Raad, Michael Stadler, Eva M. Weissinger, Ernst Holler, Danny Jonigk, D Ihlenburg-Schwarz, Zoya Kuzmina |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oncology Adult Male Proteomics Cancer Research medicine.medical_specialty Adolescent Proteome Urinary system medicine.medical_treatment Graft vs Host Disease Hematopoietic stem cell transplantation Severity of Illness Index Cohort Studies 03 medical and health sciences Young Adult 0302 clinical medicine immune system diseases hemic and lymphatic diseases Internal medicine Severity of illness medicine Odds Ratio Cluster Analysis Humans Transplantation Homologous Aged Hematology business.industry Incidence Hematopoietic Stem Cell Transplantation Reproducibility of Results Odds ratio Middle Aged medicine.disease Lymphoma Transplantation Leukemia 030104 developmental biology ROC Curve 030220 oncology & carcinogenesis Immunology Chronic Disease Female business Peptides |
| Zdroj: | Leukemia. 31(3) |
| ISSN: | 1476-5551 |
| Popis: | Allogeneic hematopoietic stem cell transplantation (allo-HSCT) may be curative, but is associated with significant morbidity and mortality. Chronic graft-versus-host disease (cGvHD), characterized by inflammation and fibrosis of multiple target organs, considerably contributes to the morbidity and mortality even years after allo-HSCT. Diagnosis of cGvHD is based on clinical features and histology of biopsies. Here, we report the generation of a urinary cGvHD-specific proteome-pattern (cGvHD_MS14) established by capillary electrophoresis-mass spectrometry to predict onset and severity of cGvHD as an unbiased laboratory test. cGvHD_MS14 was evaluated on samples from 412 patients collected prospectively in four transplant centers. Sensitivity and specificity was 84 and 76% by cGvHD_MS14 classification. Sensitivity further increased to 93% by combination of cGvHD_MS14 with relevant clinical variables to a logistic regression model. cGvHD was predicted up to 55 days prior to clinical diagnosis. Acute GvHD is not recognized by cGvHD_MS14. cGvHD_MS14 consists of 14 differentially excreted peptides, six of those have been sequenced to date and are fragments from thymosin β-4, eukaryotic translation initiation factor 4γ2, fibrinogen β-chain or collagens. In conclusion, the cGvHD_MS14-pattern allows early, highly sensitive and specific prediction of cGvHD as an independent diagnostic criterion of clinical diagnosis potentially allowing early therapeutic intervention. |
| Databáze: | OpenAIRE |
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