CD4 T Cells Are the Only Lymphocytes Needed To Protect Mice against Rotavirus Shedding after Intranasal Immunization with a Chimeric VP6 Protein and the Adjuvant LT(R192G)
| Autor: | Richard L. Ward, John D. Clements, John L. VanCott, Anthony H.-C. Choi, Jason A. Flint, Monica M. McNeal, Susan C. Stone, Matili Basu |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
CD4-Positive T-Lymphocytes
Rotavirus Adoptive cell transfer Receptors Antigen T-Cell alpha-beta Recombinant Fusion Proteins medicine.medical_treatment T cell Lymphocyte Bacterial Toxins Immunology Mice Nude CD8-Positive T-Lymphocytes Biology Microbiology Rotavirus Infections Enterotoxins Mice Capsid Adjuvants Immunologic Antigen Immunity Virology Vaccines and Antiviral Agents medicine Animals Cytotoxic T cell Antigens Viral Immunity Mucosal Administration Intranasal B-Lymphocytes Mice Inbred BALB C Escherichia coli Proteins Immunologic Deficiency Syndromes Flow Cytometry Adoptive Transfer DNA-Binding Proteins Mice Inbred C57BL medicine.anatomical_structure Immunization Insect Science Capsid Proteins Adjuvant Gene Deletion |
| Zdroj: | Journal of Virology. 76:560-568 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.76.2.560-568.2002 |
| Popis: | Intranasal immunization of mice with a chimeric VP6 protein and the mucosal adjuvantEscherichia coliheat labile toxin LT(R192G) induces nearly complete protection against murine rotavirus (strain EDIM [epizootic diarrhea of infant mice virus]) shedding for at least 1 year. The aim of this study was to identify the protective lymphocytes elicited by this new vaccine candidate. Immunization of mouse strains lacking one or more lymphocyte populations revealed that protection was dependent on αβ T cells but mice lacking γδ T cells and B cells remained fully protected. Furthermore, depletion of CD8 T cells in immunized B-cell-deficient mice before challenge resulted in no loss of protection, while depletion of CD4 T cells caused complete loss of protection. Therefore, αβ CD4 T cells appeared to be the only lymphocytes required for protection. As confirmation, purified splenic T cells from immunized mice were intraperitoneally injected into Rag-2 mice chronically infected with EDIM. Transfer of 2 × 106CD8 T cells had no effect on shedding, while transfer of 2 × 105CD4 T cells fully resolved shedding in 7 days. Interestingly, transfer of naive splenic CD4 T cells also resolved shedding but more time and cells were required. Together, these results establish CD4 T cells as effectors of protection against rotavirus after intranasal immunization of mice with VP6 and LT(R192G). |
| Databáze: | OpenAIRE |
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