Persistent organic pollutants alter DNA methylation during human adipocyte differentiation

Autor: Albertinka J. Murk, Dieuwertje E. Kok, Myrthe W. van den Dungen, Wilma T. Steegenga
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Perfluorooctanesulfonic acid
Polychlorinated Dibenzodioxins
Nutrition and Disease
010501 environmental sciences
Toxicology
01 natural sciences
Human mesenchymal stem cells
chemistry.chemical_compound
Voeding
Metabolisme en Genomica
Adipocyte
Voeding en Ziekte
Gene expression
Adipocytes
Cells
Cultured
Fluorocarbons
Adipogenesis
DNA methylation
Cell Differentiation
General Medicine
Phenotype
Metabolism and Genomics
Cell biology
Biochemistry
Alkanesulfonic Acids
Metabolisme en Genomica
Nutrition
Metabolism and Genomics
Environmental Pollutants
Milieutechnologie
Cell Survival
Biology
03 medical and health sciences
Marine Animal Ecology
Voeding
Humans
Persistent organic pollutants (POPs)
Gene
0105 earth and related environmental sciences
VLAG
Nutrition
Mesenchymal stem cell
Infinium 450K BeadChip
Mesenchymal Stem Cells
Mariene Dierecologie
030104 developmental biology
chemistry
Environmental Technology
Trialkyltin Compounds
Transcriptome
Zdroj: Toxicology in Vitro 40 (2017)
Toxicology in Vitro, 40, 79-87
ISSN: 0887-2333
Popis: Ubiquitous persistent organic pollutants (POPs) can accumulate in humans where they might influence differentiation of adipocytes. The aim of this study was to investigate whether DNA methylation is one of the underlying mechanisms by which POPs affect adipocyte differentiation, and to what extent DNA methylation can be related to gene transcription. Adipocyte differentiation was induced in two human cell models with continuous exposure to different POPs throughout differentiation. From the seven tested POPs, perfluorooctanesulfonic acid (PFOS) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) decreased lipid accumulation, while tributyltin (TBT) increased lipid accumulation. In human mesenchymal stem cells (hMSCs), TCDD and TBT induced opposite gene expression profiles, whereas after PFOS exposure gene expression remained relatively stable. Genome-wide DNA methylation analysis showed that all three POPs affected DNA methylation patterns in adipogenic and other genes, possibly related to the phenotypic outcome, but without concomitant gene expression changes. Differential methylation was predominantly detected in intergenic regions, where the biological relevance of alterations in DNA methylation is unclear. This study demonstrates that POPs, at environmentally relevant levels, are able to induce differential DNA methylation in human differentiating adipocytes.
Databáze: OpenAIRE
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