Axl-/- mice have delayed recovery and prolonged axonal damage following cuprizone toxicity
| Autor: | Hannah J. Hoehn, Sarah Bourdon, Bridget Shafit-Zagardo, Celia F. Brosnan, Yvonne Kress, Albert Sohn |
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| Rok vydání: | 2008 |
| Předmět: |
Ratón
Apoptosis Mice Transgenic Biology Corpus Callosum Andrology chemistry.chemical_compound Myelin Cuprizone Mice Microscopy Electron Transmission Proto-Oncogene Proteins medicine In Situ Nick-End Labeling Oil Red O Animals Molecular Biology Chelating Agents Oncogene Proteins GAS6 General Neuroscience Receptor Protein-Tyrosine Kinases Axl Receptor Tyrosine Kinase Oligodendrocyte Axons Oligodendroglia medicine.anatomical_structure chemistry Microscopy Fluorescence Immunology Toxicity Neuroglia Neurology (clinical) Developmental Biology |
| Zdroj: | Brain research. 1240 |
| ISSN: | 1872-6240 |
| Popis: | Activation of the receptor tyrosine kinase Axl recruits signaling molecules that regulate cell growth and survival. To evaluate Axl's role in brain during cuprizone toxicity, mice were fed cuprizone and evaluated at 3-, 4-, and 6-week cuprizone treatment and 3- and 5-week post-cuprizone withdrawal. At 4-week cuprizone treatment, the corpora callosa of wildtype (WT) mice had robust Oil Red O+ staining indicative of ongoing phagocytosis. Axl-/- mice had minimal Oil Red O+ staining, fewer microglia, and significantly more TUNEL+/ASPA+ mature oligodendrocytes than the WT. At 6-week cuprizone treatment, there was significantly more Oil Red O+ staining in the Axl-/- corpora callosa than in the WT indicating a lag in the clearance of cellular and myelin debris. Relative to WT mice, there were fewer mature oligodendrocytes and significantly more SMI-32+ axons at 3-week post-cuprizone withdrawal, indicative of axonal damage in the Axl-/- corpora callosa. Electron microscopy determined that at 3-week post-cuprizone withdrawal the number of dystrophic axons and axons containing autophagosome-like vacuoles/mouse was increased in the Axl-/- mice relative to the WT mice. In Axl-/- corpora callosa, 5-week post-cuprizone withdrawal, the number of mature oligodendrocytes was comparable to the WT mice, but axons in the Axl-/- mice were SMI-32+, suggesting that Axl-/- mice have delayed clearance of apoptotic oligodendrocytes and myelin debris resulting in prolonged axonal damage and recovery from cuprizone toxicity. |
| Databáze: | OpenAIRE |
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