Amphipathic variable region heavy chain peptides derived from monoclonal human Wegener's anti-PR3 antibodies stimulate lymphocytes from patients with Wegener's granulomatosis and microscopic polyangiitis
| Autor: | C. Myers, Ralph C. Williams, Elena Csernok, R. Staud, C C Malone, Wolfgang L. Gross, Ammon B. Peck, E. Peen |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
Vasculitis Cellular immunity Myeloblastin Lymphocyte Molecular Sequence Data Immunology Immunoglobulin Variable Region Epitopes T-Lymphocyte Lymphocyte Activation medicine.disease_cause Epitope Antibodies Antineutrophil Cytoplasmic Autoimmunity Rheumatic Disease/Vasculitis medicine Humans Immunology and Allergy Amino Acid Sequence Cells Cultured Aged biology Serine Endopeptidases Granulomatosis with Polyangiitis Antibodies Monoclonal Middle Aged medicine.disease medicine.anatomical_structure Peripheral blood lymphocyte Monoclonal biology.protein Antibody Immunoglobulin Heavy Chains Peptides Microscopic polyangiitis |
| Zdroj: | Clinical and Experimental Immunology. 125:323-331 |
| ISSN: | 1365-2249 0009-9104 |
| Popis: | SummaryAmphipathic variable-region heavy chain 11-mer peptides from monoclonal human IgM antiproteinase-3 antibodies were studied for peripheral blood lymphocyte stimulation in 21 patients with Wegener's granulomatosis (WG) or microscopic polyangiitis (MPA), connective tissue disease controls and normal control subjects. Positive T-cell activation was observed in most experiments with WG patients' lymphocytes using amphipathic VH-region peptides from four different human monoclonal anti-PR3 antibodies. Control peptides of the same length but without amphipathic characteristics along with other amphipathic peptides not derived from monoclonal anti-PR3 sequence were employed as controls. No significant lymphocyte stimulation was observed with normal controls, but positive stimulation with amphipathic VH peptides was also recorded in other connective tissue disease controls mainly patients with rheumatoid arthritis. Amphipathic peptides not derived from anti-PR3 sequence did not stimulate WG lymphocytes. Our findings indicate that lymphocyte reactivity as an element of cell-mediated immunity may be activated by amphipathic VH-region amino acid sequences of autoantibodies which are themselves associated with diseases such as WG. |
| Databáze: | OpenAIRE |
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