Transactivation of cellular promoters by an integrated hepatitis B virus DNA
| Autor: | S. Yamamoto, H. Nakatake, Shoko Kawamoto, R. Koshy, Kenji Matsubara, M. Takimoto |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Transcriptional Activation
Hepatitis B virus Transcription Genetic Hepatitis B virus DNA polymerase Virus Integration Molecular Sequence Data Biophysics Biology medicine.disease_cause Biochemistry Chloramphenicol acetyltransferase Transactivation medicine Tumor Cells Cultured Humans Genomic library Viral Regulatory and Accessory Proteins Amino Acid Sequence Promoter Regions Genetic Molecular Biology Terminator Regions Genetic Mutation Base Sequence Promoter Cell Biology biology.organism_classification Virology Molecular biology Hepadnaviridae DNA Viral Trans-Activators |
| Zdroj: | Biochemical and biophysical research communications. 192(1) |
| ISSN: | 0006-291X |
| Popis: | A new Hepatitis B virus (HBV) DNA integrant clone DA2-6, isolated from a human hepatocellular carcinoma (HCC) genomic library, was tested for its ability to transactivate expression of other genes. DA2-6 consists of 3.7 kb flanking cellular sequences and an integrated 2.8 kb HBV DNA which covers the region of preS, S, and the 3′ truncated X. Using a chloramphenicol acetyltransferase (CAT) assay, a number of cellular and viral promoters were transactivated by DA2-6,and the spectrum of transactivational effect was the same as that by the wild type X gene of the virus. Deletion mutant analyses indicated that the transactivation function of DA2-6 is expressed by the region that encodes a truncated X-cell fusion product. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|