Phylogenetic characterisation of feline immunodeficiency virus in naturally infected cats in Croatia indicates additional heterogeneity of subtype B in Europe
| Autor: | Zoran Milas, Zrinka Štritof, Suzana Hađina, Ljubo Barbić, Vesna Mojčec-Perko, Matko Perharić, Nenad Turk, Vladimir Stevanović, Snježana Kovač, Krešimir Martinković, Josipa Habuš, Vilim Starešina, Marina Biđin |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Feline immunodeficiency virus medicine.medical_specialty Croatia 040301 veterinary sciences Immunodeficiency Virus Feline Biology Cat Diseases Polymerase Chain Reaction Chromatography Affinity law.invention 0403 veterinary science 03 medical and health sciences Medical microbiology law Phylogenetics Virology medicine Animals Phylogeny Polymerase chain reaction CATS Phylogenetic tree Genetic Variation 04 agricultural and veterinary sciences General Medicine Group-specific antigen biology.organism_classification FIV Phylogenetic characterization Subtype B 030104 developmental biology Cats Lentivirus Infections biology.protein Antibody |
| Popis: | This study was performed on 29 domestic cats with a variety of clinical signs, possibly related to FIV infection. Blood samples were tested by a rapid immunochromatographic (ICA) procedure for detection of FIV antibodies. Subsequently, polymerase chain reaction (PCR) was performed to amplify a portion of the proviral gag gene. All 11 positive PCR products were sequenced and compared with previously reported FIV sequences. Croatian proviral isolates that could be amplified were clustered within subtype B, and additional heterogeneity was confirmed by the formation of three separate clusters. Phylogenetic analysis of circulating strains in Croatia and in southeast Europe is necessary to improve diagnostic methods and selection of the appropriate vaccinal strains. |
| Databáze: | OpenAIRE |
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