Growth and Extended Survival of Escherichia coli O157:H7 in Soil Organic Matter
| Autor: | Volker S. Brözel, Amy A Christie, Gitanjali NandaKafle, Sébastien Vilain |
|---|---|
| Přispěvatelé: | Chimie et Biologie des Membranes et des Nanoobjets (CBMN), École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Centre de Génomique Fonctionnelle de Bordeaux (CGFB) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) media_common.quotation_subject proteome 030106 microbiology Population Bacillus cereus lcsh:QR1-502 Escherichia coli O157:H7 medicine.disease_cause Microbiology survival Competition (biology) lcsh:Microbiology 03 medical and health sciences Nutrient soil organic matter medicine Organic matter Food science stationary phase education Escherichia coli 030304 developmental biology media_common Original Research 2. Zero hunger chemistry.chemical_classification 0303 health sciences education.field_of_study biology 030306 microbiology Soil organic matter Protein turnover [CHIM.MATE]Chemical Sciences/Material chemistry 15. Life on land biology.organism_classification chemistry Spinach |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Frontiers Media, In press, 9, ⟨10.3389/fmicb.2018.00762⟩ Frontiers in Microbiology, Vol 9 (2018) |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2018.00762⟩ |
| Popis: | EnterohaemorrhagicEscherichia colisuch as serotype O157:H7 are a leading cause of food-associated outbreaks. While the primary reservoir is associated with cattle, plant foods have been associated as sources of human infection.E. coliis able to grow in the tissue of food plants such as spinach. While fecal contamination is the primary suspect, soil has been underestimated as a potential reservoir. Persistence of bacterial populations in open systems is the product of growth, death, predation, and competition. Here we report thatE. coliO157:H7 can grow using the soluble compounds in soil, and characterize the effect of soil growth in the stationary phase proteome.E. coli933D (stxII-) was cultured in Soil Extracted Soluble Organic Matter (SESOM) and the culturable count determined for 24 d. The proteomes of exponential and stationary phase populations were characterized by 2D gel electrophoresis and protein spots were identified by MALDI-TOF mass spectrometry. While LB controls displayed a death phase, SESOM grown population remained culturable for 24 d, indicating an altered physiological state with superior longevity. This was not due to decreased cell density on entry to stationary phase as 24h SESOM populations concentrated 10-fold retained their longevity. Principal component analysis showed that stationary phase proteomes from SESOM and LB were different. Differences included proteins involved in stress response, motility, membrane and wall composition, nutrient uptake, translation and protein turnover, and anabolic and catabolic pathways, indicating an altered physiological state of soil-grown cells entering stationary phase. The results suggest thatE. colimay be a soil commensal that in absence of predation and competition maintains stable populations in soil. |
| Databáze: | OpenAIRE |
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