Transcriptional inhibition of the bacteriophage T7 early promoter region by oligonucleotide triple helix formation
| Autor: | Manoj Samuel, Steven L. Broitman, Celia Ross |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Base Sequence
Transcription Genetic biology Oligonucleotide Molecular Sequence Data Biophysics Promoter Templates Genetic Cell Biology biology.organism_classification Biochemistry Molecular biology Bacteriophage Kinetics Podoviridae Oligodeoxyribonucleotides In vivo Transcription (biology) Duplex (building) Bacteriophage T7 Nucleic Acid Conformation RNA Viral Promoter Regions Genetic Molecular Biology Triple helix |
| Zdroj: | Biochemical and Biophysical Research Communications. 189:1674-1680 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(92)90270-u |
| Popis: | We have identified a purine-rich triplex binding sequence overlapping a −35 transcriptional early promoter region of the bacteriophage T 7 . Triplex-forming oligonucleotide designed to bind this target was annealed to T 7 templates and introduced into in vitro transcription systems under conditions favoring specific initiation from this promoter. These templates demonstrated significant transcriptional inhibition relative to naked genomic templates and templates mixed with non-triplex-forming oligonucleotide. It is suggested that triplex formation along this target interferes with transcriptional initiation, and this mechanism may hold potential to disrupt bacteriophage T 7 early transcription in vivo . |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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