Deep sequencing of naupliar-, cyprid- and adult-specific normalised Expressed Sequence Tag (EST) libraries of the acorn barnacle Balanus amphitrite
| Autor: | J. Grant Burgess, Sven Klages, Oliver Rupp, Maria Ina Arnone, Alexander Goesmann, Michael Kube, Florian Knaust, Thomas Bekel, Tristano Bacchetti De Gregoris, Richard Reinhardt, Anthony S. Clare |
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| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
Biofouling Aquatic Science barnacles 01 natural sciences Applied Microbiology and Biotechnology Deep sequencing Balanus settlement 03 medical and health sciences Barnacle User-Computer Interface Databases Genetic Animals Adult stage development Gene 030304 developmental biology Water Science and Technology Gene Library Genetics Expressed Sequence Tags 0303 health sciences Expressed sequence tag Life Cycle Stages biology Behavior Animal Ecology 010604 marine biology & hydrobiology Thoracica 454 sequencing Sequence Analysis DNA biology.organism_classification lectins Instar Pyrosequencing |
| Zdroj: | Biofouling. 27(4) |
| ISSN: | 1029-2454 |
| Popis: | In order to improve the genetic characterisation of the barnacle Balanus amphitrite, normalised EST libraries for the developmental stages, viz. nauplius (a mix of instars I and II), cyprid and adult, were generated. The libraries were sequenced independently using 454 technologies and 575,666 reads were generated. For adults, 4843 unique isotigs were estimated and 6754 and 7506 in the cyprid and naupliar stage, respectively. It was found that some of the previously proposed cyprid-specific bcs genes were also expressed during the naupliar and adult stage. Furthermore, as lectins have been hypothesised to influence settlement cue recognition in barnacles, the database was searched for lectin-like isotigs. Two proteins, uniquely expressed in either the cyprid or the adult stage, matched a mannose receptor, and their nucleotide sequences were 33% and 31% identical to a lectin (BRA-3) isolated from Megabalanus rosa. Further characterisation of these genes may suggest their involvement in settlement. |
| Databáze: | OpenAIRE |
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