| Autor: |
Giorgio Basili, Valentina Faoro, Serena Bonin, Isabella Dotti |
| Přispěvatelé: |
Stanta G., Dotti, Isabella, Bonin, Serena, Basili, Giorgio, Faoro, Valentina |
| Jazyk: |
angličtina |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
Guidelines for Molecular Analysis in Archive Tissues ISBN: 9783642178894 |
| Popis: |
Archival formalin-fixed and paraffin-embedded (FFPE) tissues that represent the standard material used for histologic examination have recently become a valuable source for macromolecule extraction and analysis. Conventional formalin fixation, however, remarkably reduces the quality and quantity of extracted macromolecules, thus affecting the reliability of the experimental results. Moreover, there is increasing evidence that formalin represents a hazardous chemical component that requires specific precautions when used. In the meantime, several alternative fixatives have been proposed to overcome the limitations of using formalin. Among them are alcoholic and nonalcoholic solutions, home-made fixatives and commercial fixatives. In this chapter, the main characteristics of formalin-free fixatives are illustrated, focusing especially on their advantages in terms of morphological preservation, feasibility in immunohistochemical analysis, and macromolecule preservation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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