Correlation between silver-stained nucleolar organizer region area and cell cycle time
| Autor: | Marie-Paule Montmasson, Véronique Canet, Yves Usson, Gérard Brugal, Françoise Giroud |
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| Přispěvatelé: | RFMQ, Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Usson, Yves |
| Rok vydání: | 2001 |
| Předmět: |
MESH: Ki-67 Antigen
Silver Staining [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging MESH: Nucleolus Organizer Region Biophysics MESH: Cell Cycle Biology Autoantigens MESH: Silver Staining Pathology and Forensic Medicine Endocrinology Nucleolus Organizer Region Tumor Cells Cultured Doubling time Humans MESH: Tumor Cells Cultured MESH: Antigens Nuclear Image Cytometry MESH: Humans Cell Cycle MESH: Biological Markers Temperature Nuclear Proteins Antigens Nuclear Cell Biology Hematology Cell cycle MESH: Temperature Nucleolar Organizer Region Staining Cell biology [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging Ki-67 Antigen MESH: Autoantigens Ki-67 biology.protein MESH: Cell Division Interphase MESH: Nuclear Proteins Cytometry MESH: Image Cytometry Biomarkers Cell Division |
| Zdroj: | Cytometry / Cytometry A; Cytometry B Clin Cytom; Cytometry B Clin Cytometry Cytometry / Cytometry A; Cytometry B Clin Cytom; Cytometry B Clin Cytometry, 2001, 43 (2), pp.110-6 |
| ISSN: | 0196-4763 |
| Popis: | Background The relationship between the population doubling time and the quantity of silver-stained nucleolar organizer region (AgNOR) interphase proteins was studied in cell culture at three different temperatures used to modulate the cell cycle duration. Methods After MIB 1 and AgNOR combined staining, the quantity of AgNOR proteins was measured in cycling cells by image cytometry. Results Among the several parameters calculated, the AgNOR relative area showed a strong correlation with the changes of the population doubling time induced by different temperatures. Conclusions The results support the hypothesis that the cell cycle time and the size of the ribogenesis machinery are coregulated and that measurements of AgNORs can thus be used as a static evaluation of the cell cycle duration in arbitrary units. Cytometry 43:110–116, 2001. © 2001 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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