Evaluation of a platelet apheresis technique for the preparation of leukocyte-reduced platelet concentrates
| Autor: | Geert van Waeg, Jürgen Zingsem, Anke Glaser, Reinhold Eckstein, Robert Zimmermann, Bernd Neidhardt, Volker Weisbach |
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| Rok vydání: | 1998 |
| Předmět: |
Quality Control
business.industry Platelet Count Plateletpheresis Centrifugation Hematology General Medicine Platelet Transfusion Lymphocyte Depletion Cell sorter Leukocyte Count Apheresis Platelet transfusion medicine.anatomical_structure Evaluation Studies as Topic White blood cell Immunology Platelet apheresis medicine Humans Platelet Prospective Studies business Software |
| Zdroj: | Vox sanguinis. 74(3) |
| ISSN: | 0042-9007 |
| Popis: | Reduction of the white blood cell (WBC) contamination in platelet concentrates (PC) protects patients from the immunological and infectious side effects of platelet transfusion caused by WBC. This can be done either by filtration of the PC or by improved apheresis techniques that yield WBC-poor preparations.To evaluate an improved technique for platelet collection, we carried out 201 separations in 89 healthy cytapheresis donors using the new COBE Spectra leukoreduction system (LRS) and compared the results with those of standard dual-needle separations obtained with the same cell separator.A small but statistically significant difference was found in platelet collection efficiency in comparison with the standard non-LRS software procedures (LRS: 52.6 vs. 56.3% for the reference). However, median WBC contamination was only 0.01 x 10(6) WBC per LRS product. This significant (p0.0005) improvement corresponds to a 10-fold reduction of WBC as compared with the standard dual-needle technique.The COBE Spectra LRS system produced PCs with a platelet collection efficiency nearly equal to previous techniques and with a residual WBC content satisfying even the most stringent criteria for WBC-depleted blood components. As this purity is achieved without important platelet loss, conventional fiber filtration no longer seems necessary in this kind of PC. |
| Databáze: | OpenAIRE |
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