Association between FTO polymorphism in exon 3 with carcass and meat quality traits in crossbred ducks
| Autor: | N. N. Zhang, Liang Li, X.P. Xiong, Q. Song, D.M.C. Wang, W. Gan |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Candidate gene Linkage disequilibrium Meat Molecular Sequence Data Single-nucleotide polymorphism Biology Crossbreed Polymorphism Single Nucleotide Linkage Disequilibrium Avian Proteins Animal science Quantitative Trait Heritable Gene Frequency Genotype Genetics Animals Obesity Muscle Skeletal Molecular Biology Crosses Genetic Genetic association Base Sequence Haplotype food and beverages General Medicine Exons Sequence Analysis DNA Ducks Adipose Tissue Haplotypes Mutation Body Composition Female Intramuscular fat |
| Zdroj: | Genetics and molecular research : GMR. 14(2) |
| ISSN: | 1676-5680 |
| Popis: | The fat mass and obesity-associated gene (FTO) is an excellent candidate gene that affects energy metabolism. Single nucleotide polymorphisms (SNPs) in FTO are associated with carcass and meat quality traits in pigs, cattle, and rabbits. The aim of this study was to investigate the association between novel SNPs in the FTO coding region and carcass and meat quality traits in 95 crossbred ducks, using DNA sequencing. We found two transitions G/A (SNP 387 and 473) within exon 3. SNP 387 was a synonymous mutation, whereas SNP 473 was a missense mutation. Association analysis suggested that SNP g.387GA was significantly associated with all of the carcass traits measured, the intramuscular fat content (IMF), cooking yield (CY), pH values 45 min after slaughter (pH45m), drip losses from the breast muscle, and the leg muscle (P0.05). For SNP g.473GA, the genotype AA exhibited greater leg muscle weight than the genotypes GG or AG (P0.05). The D value suggested that the two SNPs exhibited strong linkage disequilibrium. Three haplotypes (G1G2, G1A2, and A1A2) were significantly associated with IMF, CY, the a* value, and all of the carcass traits measured (P0.05). The results suggest that FTO is a candidate locus that affects carcass and meat quality traits in ducks. |
| Databáze: | OpenAIRE |
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