Atrap Deficiency Increases Arterial Blood Pressure and Plasma Volume
| Autor: | Hayo Castrop, Bernhard Gess, Frank Schweda, Mona Oppermann |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Hypertension Renal Consciousness Renal cortex Gene Expression Blood Pressure Plasma renin activity Receptor Angiotensin Type 1 Iodine Radioisotopes Kidney Tubules Proximal chemistry.chemical_compound Mice Internal medicine Renin–angiotensin system Renin medicine Animals Telemetry Vasoconstrictor Agents Plasma Volume Aldosterone Adaptor Proteins Signal Transducing Kidney Angiotensin II receptor type 1 Dose-Response Relationship Drug Angiotensin II Isoproterenol General Medicine Juxtaglomerular apparatus Adrenergic beta-Agonists Juxtaglomerular Apparatus Mice Mutant Strains medicine.anatomical_structure Endocrinology Basic Research chemistry Nephrology Female |
| Popis: | The angiotensin receptor-associated protein (Atrap) interacts with angiotensin II (AngII) type 1 (AT1) receptors and facilitates their internalization in vitro , but little is known about the function of Atrap in vivo . Here, we detected Atrap expression in several organs of wild-type mice; the highest expression was in the kidney where it localized to the proximal tubule, particularly the brush border. There was no Atrap expression in the renal vasculature or juxtaglomerular cells. We generated Atrap-deficient (Atrap−/−) mice, which were viable and seemed grossly normal. Mean systolic BP was significantly higher in Atrap−/− mice compared with wild-type mice. Dose-response relationships of arterial BP after acute AngII infusion were similar in both genotypes. Plasma volume was significantly higher and plasma renin concentration was markedly lower in Atrap−/− mice compared with wild-type mice. 125 I-AngII binding showed enhanced surface expression of AT1 receptors in the renal cortex of Atrap−/− mice, accompanied by increased carboanhydrase-sensitive proximal tubular function. In summary, Atrap−/− mice have increased arterial pressure and plasma volume. Atrap seems to modulate volume status by acting as a negative regulator of AT1 receptors in the renal tubules. |
| Databáze: | OpenAIRE |
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