A modification of the tannic acid-phosphomolybdic acid-dye stain for demonstrating myoepithelial cells in formalin fixed tissue
| Autor: | McAuliffe Wg, Schrodt Gr, Kellogg A |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Phloxine
Bronchi Salivary Glands chemistry.chemical_compound Acetic acid Fixatives Mammary Glands Animal Formaldehyde Tannic acid Animals Humans Phosphoric Acids Molybdenum Chromatography Staining and Labeling Chemistry Myoepithelial cell Lacrimal Apparatus Epithelial Cells Hydrolyzable Tannins Staining Sweat Glands Distilled water Amido Black Phosphomolybdic acid Autopsy Anatomy |
| Zdroj: | Stain technology. 61(4) |
| ISSN: | 0038-9153 |
| Popis: | A modified tannic acid-phosphomolybdic acid-dye procedure is used for staining myoepithelial cells in formalin fixed surgical and autopsy material. Paraffin sections are brought to water, mordanted for 1 hr in Bouin's fixative previously heated to 56 C, cooled while still in Bouin's, rinsed in tap water until sections are colorless, rinsed in distilled water, treated with 5% aqueous tannic acid 5-20 min, rinsed in distilled water 30 sec or less, treated with 1% aqueous phosphomolybdic acid 10-15 min, rinsed 30 sec in distilled water, rinsed in methanol, stained 1 hr in a saturated solution of amido black or phloxine B in 9:1 methanol:acetic acid, rinsed in 9:1 methanol:acetic acid, dehydrated, cleared and mounted. Myoepithelial cells of sweat, lacrimal, salivary, bronchial, and mammary glands are blue-green with amido black or pink with phloxine B. Fine processes of myoepithelial cells are well delineated. Background staining is minimal and the procedure is highly reproducible. |
| Databáze: | OpenAIRE |
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