Immunohistochemical and Ultrastructural Analyses of Cytoplasmic Blood Plasma Inclusions of Rat Hepatocytes
| Autor: | Takaki Seki, Tomoaki Tochitani, Mami Kouchi, Izumi Matsumoto, Kaoru Toyosawa, Yoshiko Michimae, Takatoshi Koujitani, Hitoshi Funabashi |
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| Rok vydání: | 2011 |
| Předmět: |
spontaneous lesions
Pathology medicine.medical_specialty Cytoplasmic inclusion business.industry Albumin blood plasma inclusions Case Report Periodic acid–Schiff stain liver Toxicology Pathology and Forensic Medicine rats Cytoplasm Toxicity Blood plasma Ultrastructure Immunohistochemistry Medicine business |
| Zdroj: | Journal of Toxicologic Pathology |
| ISSN: | 1881-915X 0914-9198 |
| DOI: | 10.1293/tox.24.245 |
| Popis: | In the present study, we investigated the histological, immunohistochemical and ultrastructural characteristics of cytoplasmic blood plasma inclusions that spontaneously occurred in a rat liver. Histologically, a number of cytoplasmic inclusions were observed in the liver of an 8-week-old female SD rat. These inclusions were strongly positive for PAS staining and resistant to diastase digestion. Immunohistochemical analyses revealed that these inclusions were positive for albumin and IgG; however, most of them were negative for LAMP-1 and LAMP-2. Ultrastructurally, the inclusions were surrounded by limiting membranes and composed of moderately electron dense, homogenous materials. These characteristics described here represent valuable information for pathological examination in toxicity studies. |
| Databáze: | OpenAIRE |
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