The Relationship between Transport-enhancement Effects and Cell Viability by Capric Acid Sodium Salt, Monocaprin, and Dicaproin
| Autor: | Motohiro Shima, Yukitaka Kimura, Ryuichi Matsuno, Shuji Adachi |
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| Rok vydání: | 2016 |
| Předmět: |
Chromatography
Organic Chemistry Biological activity General Medicine Biology medicine.disease Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry chemistry.chemical_compound chemistry Caco-2 Capric Acid Permeability (electromagnetism) medicine MTT assay Viability assay Diglyceride Molecular Biology Cell damage Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 62(1) |
| ISSN: | 0916-8451 |
| Popis: | Using Caco-2 cell monolayers and MTT assay, the relationship between cell viability (a) and transport-enhancement effect of 1,2-dicaproin (C6DG), monocaprin (C10MG), and capric acid sodium salt (C10FANa) was examined. Transport enhancement effect was assessed by apparent permeability (P app) of penicillin V. There was a linear relationship between (P app-aS a) and (1-a) values, where S a was the apparent permeability for the viable cells. The apparent permeability for the damaged cells (S d) was evaluated from the slope of the line. Each of the enhancer compounds gave a different S d value 2.00×10(-4), 0.82×10(-4), and 0.10×10(-4) cm/s for C6DG, C10MG, and C10FANa, respectively, but the value was independent of its concentration for C10MG and C10FANa. C6DG would be the safest enhancer among the three compounds because of its high S d value at the low level of cell damage. S d could be used as a criterion for estimating the safety of enhancers. |
| Databáze: | OpenAIRE |
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