Effects of nucleotide changes on the ability of hepatitis delta virus to transcribe, process, and accumulate unit-length, circular RNA
| Autor: | H J Netter, David W. Lazinski, John M. Taylor, Ting-Ting Wu |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Time Factors
Transcription Genetic Molecular Sequence Data Immunology Mutant Microbiology Cell Line Terminal loop Open Reading Frames Transcription (biology) Circular RNA Virology Animals Humans Hepatitis delta Antigens Messenger RNA Base Sequence biology Hepatitis Antigens Ribozyme Wild type RNA Molecular biology Mutagenesis Insect Science COS Cells biology.protein Nucleic Acid Conformation RNA Viral Hepatitis Delta Virus Research Article |
| Zdroj: | Journal of Virology. 71:5408-5414 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.71.7.5408-5414.1997 |
| Popis: | The circular RNA genome of hepatitis delta virus (HDV) can fold into an unbranched rodlike structure. We mutagenized the two ends of this structure and assayed the effects on the ability of the genomes to replicate and accumulate processed RNA transcripts in transfected cells. The top end, defined as that nearest to the 5' end of the putative mRNA for delta antigen, was much more sensitive than the other end, defined as the bottom. Most of the 22 mutants made at the bottom were able to accumulate RNA as well as the wild type. For deletions extending as close as 2 nucleotides (nt) from the predicted domains needed for the two ribozymes, the accumulation levels dropped to |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|