LPS-binding protein and CD14-dependent attachment of hepatitis B surface antigen to monocytes is determined by the phospholipid moiety of the particles
| Autor: | Julián Gómez-Gutiérrez, Peter Vanlandschoot, Ali Farhoudi, Francisco Gavilanes, Freya Van Houtte, Darell L. Peterson, Felix Stelter, Annelies Roobrouck, Geert Leroux-Roels |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
HBsAg
CD14 Lipopolysaccharide Receptors Phospholipid CHO Cells Biology Transfection medicine.disease_cause Monocytes law.invention Cell membrane chemistry.chemical_compound law Cricetinae Virology medicine Animals Humans Cells Cultured Phospholipids Phosphatidylglycerol Hepatitis B virus Hepatitis B Surface Antigens Membrane Glycoproteins Cell Membrane Phosphatidylserine Recombinant Proteins medicine.anatomical_structure chemistry Biochemistry Recombinant DNA Carrier Proteins Acute-Phase Proteins Protein Binding |
| Zdroj: | Journal of General Virology. 83:2279-2289 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-83-9-2279 |
| Popis: | It was observed recently that recombinant yeast-derived hepatitis B surface antigen (rHBsAg) particles, which contain the S protein only, bind almost exclusively to monocytes. It is shown here that binding requires the presence of the LPS receptor CD14. Furthermore, evidence is presented that a domain on CD14 that is identical to or largely overlaps with the LPS-binding pocket is instrumental for the attachment of rHBsAg. Additionally, it is shown that the heat-labile LPS-binding protein (LBP) catalyses the binding of rHBsAg to the cells. Remarkably, natural plasma-derived HBsAg (pHBsAg) does not have this property. pHBsAg devoid of its lipids and reconstituted with phosphatidylserine or phosphatidylglycerol acquires the characteristic of yeast-derived HBsAg. Clearly, the interaction of rHBsAg with the cell membrane is determined by the presence of charged phospholipids that are absent in pHBsAg. Although a lipid–receptor interaction is suggested, antibody-inhibition experiments suggest a possible involvement of the C-terminal region of the S protein in the interaction with monocytes. The possible implications of these observations for hepatitis B virus (HBV) infection and HBV vaccine efficiency are discussed. |
| Databáze: | OpenAIRE |
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