Characterization of genetic diversity and clonal complexes by whole genome sequencing of Mycobacterium tuberculosis isolates from Jalisco, Mexico
| Autor: | Juan Carlos Villanueva-Arias, Roberto Zenteno-Cuevas, Natividad Ayala-Chavira, Ikuri Alvarez-Maya, Luis Alfredo Rendón-Bautista, Manuel Sandoval-Díaz |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male 0301 basic medicine Microbiology (medical) Tuberculosis Lineage (genetic) Adolescent 030106 microbiology Immunology Antitubercular Agents Microbial Sensitivity Tests Biology Microbiology Mycobacterium tuberculosis Young Adult 03 medical and health sciences Drug Resistance Multiple Bacterial Tuberculosis Multidrug-Resistant Isoniazid medicine Humans Indel Mexico Phylogeny Whole genome sequencing Genetics Molecular Epidemiology Genetic diversity Whole Genome Sequencing Phylogenetic tree Middle Aged medicine.disease biology.organism_classification Phenotype 030104 developmental biology Infectious Diseases Female Rifampin Rifampicin medicine.drug |
| Zdroj: | Tuberculosis. 129:102106 |
| ISSN: | 1472-9792 |
| DOI: | 10.1016/j.tube.2021.102106 |
| Popis: | Whole genome sequencing (WGS) analysis in tuberculosis allows the prediction of drug-resistant phenotypes, identification of lineages, and to better understanding of the epidemiology and transmission chains. Nevertheless the procedure has been scarcely assessed in Mexico, in this work we analyze by WGS isolates of Mycobacterium tuberculosis circulating in Jalisco, Mexico. Lineage and phylogenetic characterization, drug resistant prediction, "in silico" spoligotyping determination, were provided by WGS in 32 M. tuberculosis clinical isolates. Lineage 4 (L4), with 28 isolates (87%) and eleven sublineages was dominant. Forty SNPs and INDELs were found in genes related to first-, and second-line drugs. Eleven isolates were sensitive, seven (22%) were predicted to be resistant to isoniazid, two resistant to rifampicin (6%) and two (6%) were multidrug-resistant tuberuclosis. Spoligotyping shows that SIT 53 (19%) and SIT 119 (16%) were dominant. Four clonal transmission complexes were found. This is the first molecular epidemiological description of TB isolates circulating in western Mexico, achieved through WGS. L4 was dominant and included a high diversity of sublineages. It was possible to track the transmission route of two clonal complexes. The WGS demonstrated to be of great utility and with further implications for clinical and epidemiological study of TB in the region. |
| Databáze: | OpenAIRE |
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