Sialic acid-binding lectin (leczyme) induces apoptosis to malignant mesothelioma and exerts synergistic antitumor effects with TRAIL
| Autor: | Takashi Omoto, Kazuo Nitta, Sen-itiroh Hakomori, Shigeki Sugawara, Takeo Tatsuta, Kohta Takahashi, Masahiro Hosono, Yukiko Kariya |
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| Rok vydání: | 2013 |
| Předmět: |
leczyme
Mesothelioma Cancer Research Lung Neoplasms synergistic antitumor effect Blotting Western Cell TRAIL Apoptosis Sialic acid binding Biology Amphibian Proteins Bid TNF-Related Apoptosis-Inducing Ligand Ribonucleases Lectins Tumor Cells Cultured medicine Humans Cytotoxicity Cell Proliferation Membrane Potential Mitochondrial Oncogene Cell growth Mesothelioma Malignant Drug Synergism Articles Cell cycle medicine.disease Molecular biology Mitochondria respiratory tract diseases Drug Combinations medicine.anatomical_structure Oncology malignant mesothelioma Cancer research lectin ribonuclease BH3 Interacting Domain Death Agonist Protein |
| Zdroj: | International Journal of Oncology |
| ISSN: | 1791-2423 1019-6439 |
| DOI: | 10.3892/ijo.2013.2192 |
| Popis: | Malignant mesothelioma is a highly aggressive tumor with poor prognosis. An effective drug for treatment of malignant mesothelioma is greatly needed. Sialic acid-binding lectin (SBL) isolated from oocytes of Rana catesbeiana is a multifunctional protein which has lectin activity, ribonuclease activity and antitumor activity, so it could be developed as a new type of anticancer drug. The validity of SBL for treatment of malignant mesothelioma was assessed using three malignant mesotheliomas and a non-malignant mesothlial cell line. Effectiveness of combinatorial treatment of SBL and tumor necrosis factor-related apoptosis inducing ligand (TRAIL) was also elucidated and characterized. SBL induced tumor-selective cytotoxicity that was attributed to induction of apoptosis. Combinatorial treatment of SBL and TRAIL showed synergistic apoptosis-inducing effect. Additional experiments revealed that Bid was the mediating molecule for the synergistic effect in SBL and TRAIL. These results suggested that SBL could be a promising candidate for the therapeutics for malignant mesothelioma. Furthermore, the combinatorial treatment of SBL and TRAIL could be an effective regimen against malignant mesothelioma. |
| Databáze: | OpenAIRE |
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