Adoptive transfer of dendritic cells expressing CD11c reduces the immunological response associated with experimental colitis in BALB/c mice
| Autor: | Fernanda Guimarães Drummond e Silva, Aureo T. Yamada, Lisiery Negrini Paiatto, Patricia Ucelli Simioni, Wirla Maria da Silva Cunha Tamashiro |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Estadual de Campinas (UNICAMP), Federal University of Ouro Preto, FAM |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adoptive cell transfer Physiology Cell- and Tissue-Based Therapy lcsh:Medicine T-Lymphocytes Regulatory Immune tolerance Mice 0302 clinical medicine Immune Physiology Cellular types Medicine and Health Sciences Medicine lcsh:Science Innate Immune System Multidisciplinary biology Immune cells Regulatory T cells Colitis Adoptive Transfer medicine.anatomical_structure Cytokines White blood cells Biological Cultures Research Article Cell biology Blood cells Ovalbumin T cell Immunology T cells CD11c Spleen Gastroenterology and Hepatology Research and Analysis Methods BALB/c 03 medical and health sciences Immune Tolerance Animals Humans Molecular Biology Techniques Molecular Biology CD86 business.industry Inflammatory Bowel Disease lcsh:R Biology and Life Sciences Dendritic Cells Molecular Development Cell Cultures medicine.disease biology.organism_classification CD11c Antigen 030104 developmental biology Trinitrobenzenesulfonic Acid Animal cells Immune System Clinical Immunology lcsh:Q B7-2 Antigen Clinical Medicine business Developmental Biology Cloning 030215 immunology |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP PLoS ONE, Vol 13, Iss 5, p e0196994 (2018) PLoS ONE |
| Popis: | Made available in DSpace on 2018-12-11T16:53:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-05-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Introduction In addition to conventional therapies, several new strategies have been proposed for modulating autoimmune diseases, including the adoptive transfer of immunological cells. In this context, dendritic cells (DCs) appear to be one of the most promising treatments for autoimmune disorders. The present study aimed to evaluate the effects of adoptive transfer of DCs obtained from both naïve and ovalbumin (OVA)-tolerant mice on the severity of TNBS induced colitis and analyze the eventual protective mechanisms. Methods and results To induce oral tolerance, BALB/c mice were fed 4mg/mL OVA solution for seven consecutive days. Spleen DCs were isolated from tolerant (tDC) and naïve (nDC) mice, and then adop-tively transferred to syngeneic mice. Three days later, colitis was induced in DC treated mice by intrarectal instillation of 100μg2,4,6-trinitrobenzenesulfonic acid (TNBS) dissolved in 50% ethanol. Control subjects received only intrarectal instillation of either TNBS solution or a vehicle. Five days later, mice from all groups were euthanized and examined for physiological and immunological parameters. Regarding the phenotype, we observed that the frequencies of CD11+ MHC II+ and CD11+ MHCII+ CD86+ cells were significantly lower in DCs isolated from tolerant mice than in those from naive mice. However, pretreatment with both types of DCs was able to significantly reduce clinical signs of colitis such as diarrhea, rectal prolapse, bleeding, and cachexia, although only treatment with tDCs was able to prevent weight loss from instillation of TNBS. In vitro proliferation of spleen cells from mice treated with either type of DCs was significantly lower than that observed in splenic cell cultures of naïve mice. Institute of Biosciences Universidade Estadual Paulista UNESP Faculty of Food Engineering University of Campinas UNICAMP Department of Food School of Nutrition Federal University of Ouro Preto Department of Biochemistry and Tissue Biology Institute of Biology University of Campinas UNICAMP Department of Genetics Evolution Microbiology and Immunology Institute of Biology University of Campinas UNICAMP Department of Biomedical Science Faculty of Americana FAM Institute of Biosciences Universidade Estadual Paulista UNESP FAPESP: 2013/20258-2 FAPESP: 2014/08591-0 FAPESP: 2014/086192 FAPESP: 2014/167010 FAPESP: 2015/09326-1 |
| Databáze: | OpenAIRE |
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